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Team:EPF-Lausanne/Protocols/1.5 steps PCR
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Revision as of 09:37, 4 September 2009
1. Do the following mixing:
| dNTP | 1 μl |
|---|---|
| 5x Buffer + MgCl2 | 10 μl |
| Primer Mix | 1 μl |
| template | 0.5 μl |
| HiFi Plus DNAp | 0.5 μl |
| dH2O | 36.5 μl |
| Final Volume | 50 μl |
