Team:EPF-Lausanne/Protocols/1.5 steps PCR

From 2009.igem.org

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Revision as of 09:44, 4 September 2009



Miniprep Protocol




1. Do the following mixing:

dNTP 1 μl
5x Buffer + MgCl2 10 μl
Primer Mix 1 μl
template 0.5 μl
HiFi Plus DNAp 0.5 μl
dH2O 36.5 μl
Final Volume 50 μl


2. Make it run 10 cycles:

1        94°C 4:00
2       94°C 0:30
3       55°C 1:00
4       72°C 2:00
5       Cycle 2-4 10 times
6       72°C 7:00
7       4°C ∞


Retrieved from "http://2009.igem.org/Team:EPF-Lausanne/Protocols/1.5_steps_PCR"