Team:TUDelft/Project
From 2009.igem.org
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=='''Our abstract'''== | =='''Our abstract'''== | ||
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===Bacterial Relay Race=== | ===Bacterial Relay Race=== | ||
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'''Research question:''' | '''Research question:''' | ||
Can we achieve cell-to-cell communication in bacteria similar to a relay race: | Can we achieve cell-to-cell communication in bacteria similar to a relay race: |
Revision as of 16:25, 5 September 2009
Page is under construction
Project Overview
Contents |
Our abstract
Bacterial Relay Race
Research question: Can we achieve cell-to-cell communication in bacteria similar to a relay race:
- Using delay device
- Using self-destructive plasmid
- Between two F+ cells
Goals:
- Build a delay device that can change the timing of transcribing the target protein
- Build a plasmid that will produce endonucleases and cut itself into pieces (i.e. destroy itself)
- Construct a modified F plasmid that will allow F+/F+ conjugation
Why?
- To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
- To generate a pulse in the expression of a certain gene
- To control the amplitude of the pulse created by the plasmid
- Because it is cool!!!
How?
Check out the research proposal page
Applications:
- Cell synchronization
- Multiple bit signals within plasmid DNA comparing to AHL signals
- …
Project Details
The Experiments
Results