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Team:Warsaw/Calendar-Main/3 September 2009
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(New page: {{WarNotebook}} <!-- do not edit above me! --> ===<div style="text-align: center;">Acquiring the internaline A gene from ''Listeria monocytogenes'' st. EDG-e genome </div>=== '''Jarek'''...) |
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* There were no visible products of digestion, even after long treatment with ethidium bromide. Propably the Clean-Up kit isn't working well. I'll try to acquire IntA gene with another PCR. | * There were no visible products of digestion, even after long treatment with ethidium bromide. Propably the Clean-Up kit isn't working well. I'll try to acquire IntA gene with another PCR. | ||
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| + | ===<div style="text-align: center;"><h3>Preparation of glycerol stocks</h3></div>=== | ||
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| + | '''<h4>Monika</h4>''' | ||
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| + | *Preparation of glycerol stocks following the prodcedure described [https://2009.igem.org/Team:Warsaw/protocols#Preparation_of_glycerol_stocks <span style="color: blue;">here</span>] | ||
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| + | plac + GFP | ||
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| + | AraC + GFP | ||
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| + | plac + RBS + cI + terminator | ||
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| + | plac + RBS + cI + RBS + GFP + terminator | ||
Revision as of 10:49, 6 September 2009
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Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome
Jarek
Tasks:
- Digestion of PCR products, separetly with MboI and CaiI restriction endonucleases
- Electrophoretical segregation of digested samples in 1,5% agarose gel
- Preparation of another PCR reaction with L.monocytogenes genome DNA
Results:
- There were no visible products of digestion, even after long treatment with ethidium bromide. Propably the Clean-Up kit isn't working well. I'll try to acquire IntA gene with another PCR.
Preparation of glycerol stocks
Preparation of glycerol stocks
Monika
- Preparation of glycerol stocks following the prodcedure described here
plac + GFP
AraC + GFP
plac + RBS + cI + terminator
plac + RBS + cI + RBS + GFP + terminator
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