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Team:Groningen/Project/Transport
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==Introduction== | ==Introduction== | ||
| - | + | We are trying to find suitable systems capable of isolating heavy metals from the environment. There are several different mechanisms to achieve such a thing. We examined 3 kinds: | |
| - | + | *Metal transporters, that transport the metal from the environment (ie. wastewater) to the cytoplasm | |
| + | **Uncoupled | ||
| + | **Coupled with 'helper' protein | ||
| + | *Metal binding proteins in the periplasm | ||
| - | We | + | We will investigate severals systems, to find which are suitable for the final design. |
| + | the following systems are under concideration. | ||
| - | + | *Copper /zinc uptake via HmtA | |
| - | + | *heavy metal uptake coupled to citrate via ''ef''CitH | |
| - | + | *Arsenite uptake via GlpF | |
| - | + | *Periplasmic accumulation of heavy metals via Mer Operon. | |
| - | + | ||
| - | + | ||
==HmtA== | ==HmtA== | ||
Revision as of 13:58, 7 June 2009
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Introduction
We are trying to find suitable systems capable of isolating heavy metals from the environment. There are several different mechanisms to achieve such a thing. We examined 3 kinds:
- Metal transporters, that transport the metal from the environment (ie. wastewater) to the cytoplasm
- Uncoupled
- Coupled with 'helper' protein
- Metal binding proteins in the periplasm
We will investigate severals systems, to find which are suitable for the final design. the following systems are under concideration.
- Copper /zinc uptake via HmtA
- heavy metal uptake coupled to citrate via efCitH
- Arsenite uptake via GlpF
- Periplasmic accumulation of heavy metals via Mer Operon.
HmtA
HmtA, heavy metal transporter A from Pseudomonas aeruginosa Q9I147 is a P-type ATPase importer. It mediates the uptake of Copper (Cu) and Zinc (Zn) and is functionally expressed in E.coli.
>gi|81857196|sp|Q9I147|Q9I147_PSEAE Probable cation-transporting P-type ATPase
| Enzyme | Number of Sites |
|---|---|
| EcoRI | 0 |
| XbaI | 0 |
| NotI | 0 |
| SpeI | 0 |
| PstI | 2 |
Missing information/To do
- Expression assesment
- Stability
- Level
- Functional assesment
- Uptake speed
- Affinity
- Electrolyte potential generating force
- Eliminate BioBrick restriction sites
Literature
Lewinson O., Lee A.T., Rees D.C. 2009. A P-type ATPase importer that discriminates between essential and toxic transition metals. PNAS. vol. 106, no. 12, p. 4677-4682.
Mer operon
Missing information
Literature
Lewinson O., Lee A.T., Rees D.C. 2009. A P-type ATPase importer that discriminates between essential and toxic transition metals. PNAS. vol. 106, no. 12, p. 4677-4682.
