Team:TUDelft/Project
From 2009.igem.org
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- | *To send multiple messages to a cell from | + | *To send multiple messages to a cell from an induced cell. |
*To test one condition and return to the original state without killing the cell or changing any of its previous genetic and physiological characteristics | *To test one condition and return to the original state without killing the cell or changing any of its previous genetic and physiological characteristics | ||
*To generate a pulse in the expression of a certain gene | *To generate a pulse in the expression of a certain gene | ||
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*Cell synchronization | *Cell synchronization | ||
- | *Multiple bit signals within plasmid DNA comparing to AHL signals | + | *Multiple bit signals within plasmid DNA comparing to single bit AHL signals |
*… | *… | ||
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Revision as of 13:14, 8 September 2009
This page is still under construction
Project Overview
Contents |
Our abstract
Bacterial Relay Race
Research question:
Can we achieve cell-to-cell communication in bacteria similar to a relay race:
- Using delay device
- Using self-destructive plasmid
- Between two F+ cells
Goals:
- Build a delay device that can change the timing of transcribing the target protein
- Build a plasmid that will produce endonucleases and cut itself into pieces (i.e. destroy itself)
- Construct a modified F plasmid that will allow F+/F+ conjugation
Why?
- To send multiple messages to a cell from an induced cell.
- To test one condition and return to the original state without killing the cell or changing any of its previous genetic and physiological characteristics
- To generate a pulse in the expression of a certain gene
- To control the amplitude of the pulse created by the plasmid
- Because it is cool!!!
How?
Check out the research proposal page.
Applications:
- Cell synchronization
- Multiple bit signals within plasmid DNA comparing to single bit AHL signals
- …
Project Details
The Experiments
Results