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Team:Warsaw/Calendar-Main/4 September 2009
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(New page: {{WarNotebook}} <!-- do not edit above me! --> ===<div style="text-align: center;">Acquiring the internaline A gene from ''Listeria monocytogenes'' st. EDG-e genome </div>=== '''Jarek'''...) |
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* There were no visible products on gel, it might happened that I accidently forget to add one of the substrates to reaction. | * There were no visible products on gel, it might happened that I accidently forget to add one of the substrates to reaction. | ||
| + | <h3><div style="text-align: center;">Assembly of endosomal detection operon</div></h3> | ||
| + | <h4>Marcin</h4> | ||
| - | + | Task 1: | |
| - | + | ||
| - | + | * Transformation of chemocompetent E. coli TOP10 strain. Construct to transform: | |
| + | ** [http://partsregistry.org/Part:BBa_B0032<span style="color: black">BBa_B0032</span>] with [http://partsregistry.org/Part:BBa_E0022<span style="color: black">BBa_E0022</span>] connected to [http://partsregistry.org/Part:BBa_B0032<span style="color: black">BBa_B0032</span>] with [http://partsregistry.org/Part:BBa_C0051<span style="color: black">BBa_C0051</span>] on [http://partsregistry.org/Part:pSB1A3<span style="color: black">pSB1A3</span>] plasmid | ||
| + | ** cro CDS on [http://partsregistry.org/Part:pSB1A3<span style="color: black">pSB1A3</span>] | ||
| + | ** Bax CDS on [http://partsregistry.org/Part:pSB1A3<span style="color: black">pSB1A3</span>] | ||
| + | Methods: | ||
| + | * Detailed protocol of transformation is described [https://2009.igem.org/Team:Warsaw/Calendar-Main/5_July_2009 here] | ||
<!-- do not remove this! --> | <!-- do not remove this! --> | ||
{{WarNotebookEnd}} | {{WarNotebookEnd}} | ||
Revision as of 16:55, 8 September 2009
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Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome
Jarek
Tasks:
- Electrophoretical segregation of PCR products from previous day in 0,8% agarosis gel
- Preparation of another PCR reaction with L.monocytogenes genome DNA
Results:
- There were no visible products on gel, it might happened that I accidently forget to add one of the substrates to reaction.
Assembly of endosomal detection operon
Marcin
Task 1:
- Transformation of chemocompetent E. coli TOP10 strain. Construct to transform:
- [http://partsregistry.org/Part:BBa_B0032BBa_B0032] with [http://partsregistry.org/Part:BBa_E0022BBa_E0022] connected to [http://partsregistry.org/Part:BBa_B0032BBa_B0032] with [http://partsregistry.org/Part:BBa_C0051BBa_C0051] on [http://partsregistry.org/Part:pSB1A3pSB1A3] plasmid
- cro CDS on [http://partsregistry.org/Part:pSB1A3pSB1A3]
- Bax CDS on [http://partsregistry.org/Part:pSB1A3pSB1A3]
Methods:
- Detailed protocol of transformation is described here
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