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Template:Team:KULeuven/4 September 2009/BlueLightReceptor
From 2009.igem.org
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# Gel extraction performed on the signals from 3/09 | # Gel extraction performed on the signals from 3/09 | ||
#* Nanodrop: 14,7 ng/µl of {{kulpart|BBa_J23101}} with a 260/280 of approx 2,12 | #* Nanodrop: 14,7 ng/µl of {{kulpart|BBa_J23101}} with a 260/280 of approx 2,12 | ||
| - | # Ligation with {{kulpart|BBa_J23101}} and {{kulpart|BBa_K238013}} | + | # Ligation with {{kulpart|BBa_J23101}} and {{kulpart|BBa_K238013}} (ligX 1) |
| - | # A new restriction digest on {{kulpart|BBa_J23101}} and {{kulpart|BBa_K238013}} with EcoRI and SpeI was performed. | + | # A new restriction digest on {{kulpart|BBa_J23101}} and {{kulpart|BBa_K238013}} with EcoRI and SpeI was performed. |
| + | # gel extraction of these new digests. {{kulpart|BBa_J23101}} was nanodropped with a concentration of 28 ng/µl. this was ligated with {{kulpart|BBa_K238013}} (ligX 2) | ||
| + | |||
| + | |||
| + | =saterday= | ||
| + | |||
| + | #electroporation of ligX 1&2 | ||
| + | #there was no growth of cells after electroporation with pSB3K3 | ||
| + | |||
| + | =sunday= | ||
| + | |||
| + | # there was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. this was all done in a dark room to avoid activation of the promotor. | ||
Revision as of 18:24, 8 September 2009
- Gel extraction performed on the signals from 3/09
- Nanodrop: 14,7 ng/µl of with a 260/280 of approx 2,12
- Ligation with and (ligX 1)
- A new restriction digest on and with EcoRI and SpeI was performed.
- gel extraction of these new digests. was nanodropped with a concentration of 28 ng/µl. this was ligated with (ligX 2)
saterday
- electroporation of ligX 1&2
- there was no growth of cells after electroporation with pSB3K3
sunday
- there was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. this was all done in a dark room to avoid activation of the promotor.
