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Team:Newcastle/Labwork/8 September 2009
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==<u>Metal Sensing Team</u>== | ==<u>Metal Sensing Team</u>== | ||
===Introduction=== | ===Introduction=== | ||
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Yesterday's lab session saw the Metal Sensing team innoculate 6 tubes of 5ml LB (plus ampicillin) with six cultures of ''DH5-alpha'' transformed with ''BBa_J33206'' in plasmid ''pSB1A2'' in the morning. By the time afternoon had arrived, the bacteria had grown to sufficient numbers; three of these cultures were then used to further inoculate 3 flasks of 50ml LB+amp. The three flasks of 50ml LB+amp + transformant ''E. coli'' cells were then placed in the orbital incubator and will take part in today's midi-prep. Meanwhile the six tubes were used in a mini-prep attempt but alas, the procedure went wrong and had to be abandoned. | Yesterday's lab session saw the Metal Sensing team innoculate 6 tubes of 5ml LB (plus ampicillin) with six cultures of ''DH5-alpha'' transformed with ''BBa_J33206'' in plasmid ''pSB1A2'' in the morning. By the time afternoon had arrived, the bacteria had grown to sufficient numbers; three of these cultures were then used to further inoculate 3 flasks of 50ml LB+amp. The three flasks of 50ml LB+amp + transformant ''E. coli'' cells were then placed in the orbital incubator and will take part in today's midi-prep. Meanwhile the six tubes were used in a mini-prep attempt but alas, the procedure went wrong and had to be abandoned. | ||
Revision as of 13:31, 9 September 2009
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Lab Work - 08/09/09
Metal Sensing Team
Introduction
Yesterday's lab session saw the Metal Sensing team innoculate 6 tubes of 5ml LB (plus ampicillin) with six cultures of DH5-alpha transformed with BBa_J33206 in plasmid pSB1A2 in the morning. By the time afternoon had arrived, the bacteria had grown to sufficient numbers; three of these cultures were then used to further inoculate 3 flasks of 50ml LB+amp. The three flasks of 50ml LB+amp + transformant E. coli cells were then placed in the orbital incubator and will take part in today's midi-prep. Meanwhile the six tubes were used in a mini-prep attempt but alas, the procedure went wrong and had to be abandoned.
Today's lab session will focus on creating midi-prepped plasmids from the inoculated 50ml LB+amp flasks as well as carry out restriction enzyme digest analysis on a sample. The samples will then be run on agarose gel by DNA gel electrophoresis.
Results
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
