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Team:Calgary/28 June 2009
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| - | * To attach biobrick required sites to <i>luxCDABE</i> sequencing, gradient PCR was performed. Forward and reverse primers were designed and a gradient PCR was set up for overnight. Pfx polymerase to used. The conditions that were used is: 94 degrees Celsius for 5 mins, 30 cycles (94 degrees Celsius for 30 secs, 65 degrees Celsius for 30 secs, 68 degrees Celsius for 6 mins), 68 degrees Celsius for 15 mins) | + | * To attach biobrick required sites to <i>luxCDABE</i> sequencing, gradient PCR was performed. Forward and reverse primers were designed and a gradient PCR was set up for overnight. Pfx polymerase to used. The conditions that were used is: 94 degrees Celsius for 5 mins, 30 cycles (94 degrees Celsius for 30 secs, 55-65 degrees Celsius for 30 secs, 68 degrees Celsius for 6 mins), 68 degrees Celsius for 15 mins) |
* Let gradient PCR running overnight. | * Let gradient PCR running overnight. | ||
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Revision as of 05:38, 10 September 2009
UNIVERSITY OF CALGARY
