CAROL
Biobrick Amplification
- To attach biobrick required sites to luxCDABE sequencing, gradient PCR was performed. Forward and reverse primers were designed and a gradient PCR was set up for overnight. Pfx polymerase to used. The conditions that were used is: 94 degrees Celsius for 5 mins, 30 cycles (94 degrees Celsius for 30 secs, 55-65 degrees Celsius for 30 secs, 68 degrees Celsius for 6 mins), 68 degrees Celsius for 15 mins)
- Let gradient PCR running overnight.
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