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Team:Cambridge/Notebook/Week9
From 2009.igem.org
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| + | ===Wet Work=== | ||
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| + | ====Threshold Devices==== | ||
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| + | Attempted the following standard assemblies: | ||
| + | |||
| + | :pBad + I746350 to B0015 | ||
| + | :pBad + I746351 to B0015 | ||
| + | :pBad + I746352 to B0015 | ||
| + | :I746351 to B0015 | ||
| + | :I746352 to B0015 | ||
| + | :I746352 to B0015 | ||
| + | |||
| + | The first three will be used to construct a complete device, with pBad as the sensor promoter and a pigment operon as the pigment-generating device. The pigment we chose for our proof of concept will be placed downstream of each of the 5 phage promoters, and then combined to give 15 combinations of activators and promoters, giving the construction below: | ||
| + | [[Image:completedevice.jpg]] | ||
| + | |||
| + | |||
| + | The second three will be used to construct a library of threshold devices which can be abstracted to black boxes with PoPS in and PoPS out: | ||
| + | [[Image:thresholddevice.jpg]] | ||
{{Template:CambridgeNewPage}} | {{Template:CambridgeNewPage}} | ||
Revision as of 18:24, 10 September 2009
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
Week 9
Monday
Tuesday
Wednesday
Wet Work
Threshold Devices
Confirmed successful ligation of pBad and I746350, I746351, I746352.
Thursday
Wet Work
Threshold Devices
Attempted the following standard assemblies:
- pBad + I746350 to B0015
- pBad + I746351 to B0015
- pBad + I746352 to B0015
- I746351 to B0015
- I746352 to B0015
- I746352 to B0015
The first three will be used to construct a complete device, with pBad as the sensor promoter and a pigment operon as the pigment-generating device. The pigment we chose for our proof of concept will be placed downstream of each of the 5 phage promoters, and then combined to give 15 combinations of activators and promoters, giving the construction below:
The second three will be used to construct a library of threshold devices which can be abstracted to black boxes with PoPS in and PoPS out:
Friday
