"
Team:Warsaw/Sources
From 2009.igem.org
| Line 66: | Line 66: | ||
<sup>6</sup> Mitochondrial targeting signal was amplified from the [http://www.clontech.com/images/pt/PT3730-5.pdf pAcGFP-mito] vector | <sup>6</sup> Mitochondrial targeting signal was amplified from the [http://www.clontech.com/images/pt/PT3730-5.pdf pAcGFP-mito] vector | ||
| - | <sup>7</sup> Plasmids carrying essential elements of mentioned secretion system were a generous gift from ... | + | <sup>7</sup> Plasmids carrying essential elements of mentioned secretion system were a generous gift from Carlos A. Guzman; [http://www.helmholtz-hzi.de/en/research/research_groups/vaccinology_and_applied_microbiology/vaccinology/ Department of Vaccinology and Applied Microbiology], Helmholtz Center for Infection Research |
{{WarFoot1}} | {{WarFoot1}} | ||
Revision as of 20:10, 19 September 2009
Sources of natural bricks
Below we present sources of bricks prepared by our team.
Invasiveness bricks
Most of bricks connected with bacteria invasiveness were acquired from following organisms:
| organism | genomic sequence details | acquired genes |
|---|---|---|
| Salmonella enterolitica ser. typhimurium LT21 | [http://www.ncbi.nlm.nih.gov/nuccore/NC_003197 NC_OO3197] | PhoP, PhoQ, MgtC promoter |
| Listeria monocytogenes EGD-E2 | [http://www.ncbi.nlm.nih.gov/nuccore/NC_003210 NC_003210] | listeriolysin, internalinA |
| Yersina enterolitica O:8 (8081)3 | [http://www.ncbi.nlm.nih.gov/nuccore/AM286415 AM286415] | invasin |
Other bricks
| name of the brick | sequence details | source |
|---|---|---|
| CDS of p53 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177016 BBa_K177016] | human4 |
| CDS of bax | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177027 BBa_K177027] | human 5 |
| mitochondrial targeting signal from the Cox gene | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177028 BBa_K177028] | human6 |
| secretion system | Escherichia coli7 |
1 Genomic DNA from this strain was a generous gift from ...
2 Genomic DNA from this strain was a generous gift from prof. Jacek Bielecki, PhD, [http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=528&b_2501=5 Laboratory of Applied Microbiology], Faculty of Biology, University of Warsaw
3 Genomic DNA from this strain was a generous gift from ...
4 Plasmid carrying p53 gene was a generous gift from Dawid Walerych, PhD, from the [http://www.iimcb.gov.pl/zylicz_lab.php Lab of Molecular Biology], International Institute of Molecular and Cell Biology in Warsaw
5 Plasmid carrying bax gene was a generous gift from Łukasz Borowski, MSc, from the [http://www.igib.uw.edu.pl/index.php?id=80 Institute of Genetics and Biotechnology], University of Warsaw.
6 Mitochondrial targeting signal was amplified from the [http://www.clontech.com/images/pt/PT3730-5.pdf pAcGFP-mito] vector
7 Plasmids carrying essential elements of mentioned secretion system were a generous gift from Carlos A. Guzman; [http://www.helmholtz-hzi.de/en/research/research_groups/vaccinology_and_applied_microbiology/vaccinology/ Department of Vaccinology and Applied Microbiology], Helmholtz Center for Infection Research
