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Team:TUDelft/Lock/Key library
From 2009.igem.org
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Based on the various above mentioned studies on the locks and keys we have developed an algorithm which could pave way to the development of a simple web based tool for getting the DNA sequences of locks and keys for a Ribosome Binding Site (RBS). Click [[Team:TUDelft/Algorithm |here]] for more information. | Based on the various above mentioned studies on the locks and keys we have developed an algorithm which could pave way to the development of a simple web based tool for getting the DNA sequences of locks and keys for a Ribosome Binding Site (RBS). Click [[Team:TUDelft/Algorithm |here]] for more information. | ||
| - | You can also directly visit the web based Lock/Key generator page here. | + | You can also directly visit the web based Lock/Key generator page [https://2009.igem.org/Team:TUDelft/RiboKeyLock_Generator here]. |
=='''Lock/Key library: New Biobricks Design'''== | =='''Lock/Key library: New Biobricks Design'''== | ||
Revision as of 21:29, 21 September 2009
Lock/Key library
As mentioned in preliminary approaches in the section RNA-based Delay: Biosynthetic AND gate, our design was based on the previous work of Isaacs F. 2004, [http://openwetware.org/wiki/IGEM:UC_Berkeley/2006 Berkeley iGEM 2006], [http://openwetware.org/wiki/IGEM:Caltech/2007 Caltech iGEM 2007] and [http://parts.mit.edu/igem07/index.php/Peking Peking iGEM 2007].However, we found that these projects were done using a single RBS. The registry of biobricks has several RBS, besides, as a advice from our modeling team, the RBS present in our constructs should be one with low affinity or “weak” in order to avoid leaky problems. Therefore, we constructed an algorithm based in the previous works which allows us to generate pairs of locks and keys for any RBS (in or outside the registry).
Lock/Key library: Algorithm
Based on the various above mentioned studies on the locks and keys we have developed an algorithm which could pave way to the development of a simple web based tool for getting the DNA sequences of locks and keys for a Ribosome Binding Site (RBS). Click here for more information.
You can also directly visit the web based Lock/Key generator page here.
Lock/Key library: New Biobricks Design
Lock/Key library: New Biobricks Design
