Virginia Commonwealth/11 June 2009
From 2009.igem.org
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|- | |- | ||
! E0240 | ! E0240 | ||
- | | Amp || | + | | Amp || 100s of colonies || no color || |
+ | |- | ||
+ | ! J23101 | ||
+ | | Amp || 1000s of colonies || Pink in color. || | ||
|- | |- | ||
! J23113 | ! J23113 | ||
- | | Amp || | + | | Amp || 100s of colonies || n/a || |
|- | |- | ||
! J23116 | ! J23116 | ||
| Amp || 100s of colonies || n/a || | | Amp || 100s of colonies || n/a || | ||
|- | |- | ||
- | ! | + | ! J23102 |
- | | Amp || | + | | Amp ||10s of colonies || Pink in color.|| |
|- | |- | ||
! R0040 | ! R0040 | ||
Line 98: | Line 101: | ||
|- | |- | ||
! R0011 | ! R0011 | ||
- | | Amp || | + | | Amp || 10s of colonies || n/a || |
|- | |- | ||
! J23100 | ! J23100 | ||
- | | Amp || 10s of colonies || | + | | Amp || 10s of colonies || slightly pink || |
|- | |- | ||
! J23103 | ! J23103 | ||
- | | Amp || | + | | Amp || low 100s of colonies || The colonies are pinkish in color because the rfp is being expressed. || |
|- | |- | ||
! J23104 | ! J23104 | ||
- | | Amp || | + | | Amp || 100s of colonies || n/a || |
|- | |- | ||
! J23105 | ! J23105 | ||
| Amp || 100s of colonies || n/a || | | Amp || 100s of colonies || n/a || | ||
|- | |- | ||
- | ! | + | ! J23107 |
- | | Amp || | + | | Amp ||1000s of colonies || n/a || |
|- | |- | ||
- | ! | + | ! J23110 |
- | | Amp || | + | | Amp ||1000s of colonies || n/a || |
|} | |} | ||
Revision as of 23:41, 11 June 2009
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Contents |
Thursday 11 June 2009
Results
- Note these were plated Wednesday evening with two groups splitting the work.
- Group 1: Craig, Clay, Afton plates
- Observations on the Controls
Control | Plate Type | Observation | ||
---|---|---|---|---|
NEB10β (+) | LB | lawn | ||
NEB10β (-) | Cm | no growth | ||
NEB10β (-) | Amp | no growth | ||
NEB10β (-) | Kan | 3 colonies | The possibilities for this are a non-homogeneous medium with very small sections capable of sustaining life or there was degradation of the Kan-selective medium. |
- Observations on the Colonies
Part No. | Plate Type | Colonies | Other Observations | |
---|---|---|---|---|
pSB 3K3 | Kan | 10s of colonies | n/a | |
E0240 | Amp | 100s of colonies | no color | |
J23101 | Amp | 1000s of colonies | Pink in color. | |
J23113 | Amp | 100s of colonies | n/a | |
J23116 | Amp | 100s of colonies | n/a | |
J23102 | Amp | 10s of colonies | Pink in color. | |
R0040 | Amp | 10s of colonies | n/a |
- Group 2: Adam, Kevin and Maria Plates
- Observations on the Colonies
Part No. | Plate Type | Colonies | Other Observations | |
---|---|---|---|---|
R0011 | Amp | 10s of colonies | n/a | |
J23100 | Amp | 10s of colonies | slightly pink | |
J23103 | Amp | low 100s of colonies | The colonies are pinkish in color because the rfp is being expressed. | |
J23104 | Amp | 100s of colonies | n/a | |
J23105 | Amp | 100s of colonies | n/a | |
J23107 | Amp | 1000s of colonies | n/a | |
J23110 | Amp | 1000s of colonies | n/a |
- Problems:
- Part J23106 was taken from well 18D, when it actually is located in well 18O.
- Part Q04511 was actually taken from well 18D
- Part R0011 was plated twice. The plate with the most colonies was used for growth overnight.
MandM 22:52, 11 June 2009 (UTC)
Tasks
- Literature Discussion
- Discussion of iGEM ideas
Wetlab
- Picking Transformed Colonies to be grown overnight for Mini-preps (5mL) and two Maxi-preps(125mL).
MandM 22:55, 11 June 2009 (UTC)