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Team:Imperial College London/Wetlab/Protocols/Cellculture
From 2009.igem.org
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| [[Team:Imperial_College_London/Wetlab/Protocols/Cellculture/LBPlates |Make LB plates]] | | [[Team:Imperial_College_London/Wetlab/Protocols/Cellculture/LBPlates |Make LB plates]] | ||
| *Making plates of LB agar for cell culturing<br> | | *Making plates of LB agar for cell culturing<br> | ||
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|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
Revision as of 16:19, 24 September 2009
Cell culture protocols
| Section | Assay | Overview and Aims |
|---|---|---|
| CC1: Miniprep | Link | * Harvest modest amounts of genomic DNA |
| CC2: Cellulase | Link | *Harvest large amount of genomic DNA |
| CC3: Cell competence | Link | *Making cells more competent to taking up DNA |
| CC4: Ligation | Link | *Ligation of different DNA constructs together< |
| CC5: Transformation (BL21) | Link | *Transformation into BL21 cells using electrical shock < |
| CC6: Transformation (Top10) | Transformation into Top10 | *Transformation into chemically competent Top10 cells < |
| CC7: Make LB plates | Make LB plates | *Making plates of LB agar for cell culturing |
| CC7: Extract DNA from registry | Extracting DNA | *Extracting the required DNA from the registry |
