Team:Imperial College London/Wetlab/Protocols/PromoterCharacterisation
From 2009.igem.org
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+ | =Calibration protocols (CA)= | ||
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! Section | ! Section | ||
! Assay | ! Assay | ||
! Overview and Aims | ! Overview and Aims | ||
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|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| <b>2.Auto-Induction</b> | | <b>2.Auto-Induction</b> | ||
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+ | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
+ | | <b>2.Auto-Induction</b> | ||
+ | | [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |IPTG ]] | ||
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+ | * Characterise Lac promoter by varying concentrations of IPTG | ||
+ | * Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell | ||
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Revision as of 10:55, 30 September 2009
Calibration protocols (CA)
Section | Assay | Overview and Aims |
---|---|---|
2.Auto-Induction | IPTG |
|
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | 2.Auto-Induction | IPTG |
- Characterise Lac promoter by varying concentrations of IPTG
- Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"