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Team:Imperial College London/Wetlab/Protocols/Cellculture
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| [[Team:Imperial_College_London/Wetlab/Protocols/ExtractingDNA |Extracting DNA]] | | [[Team:Imperial_College_London/Wetlab/Protocols/ExtractingDNA |Extracting DNA]] | ||
| * Extracting the required DNA from the registry<br> | | * Extracting the required DNA from the registry<br> | ||
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| + | | <b>8.Genome Restriction</b> | ||
| + | | [[Team:Imperial_College_London/Wetlab/Protocols/GenomePrep |Genome Prep]] | ||
| + | | * Preperation of the genomic DNA for restriction digest assay. | ||
| + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
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Revision as of 11:04, 30 September 2009
Cell culture protocols
| Section | Assay | Overview and Aims | |||
|---|---|---|---|---|---|
| CC1: Miniprep | Link | * Harvest smaller amounts of genomic DNA | |||
| CC2: Cellulase | Link | * Harvest larger amount of genomic DNA | |||
| CC3: Cell competence | Link | * Making cells more competent to taking up DNA | |||
| CC4: Ligation | Link | * Ligation of different DNA constructs together< | |||
| CC5: Transformation (BL21) | Link | * Transformation into BL21 cells using electrical shock < | |||
| CC6: Transformation (Top10) | Transformation into Top10 | * Transformation into chemically competent Top10 cells < | |||
| CC7: Make LB plates | Make LB plates | * Making plates of LB agar for cell culturing | |||
| CC7: Extract DNA from registry | Extracting DNA | * Extracting the required DNA from the registry
| 8.Genome Restriction | Genome Prep | * Preperation of the genomic DNA for restriction digest assay. |
