Team:Groningen/Modelling/Characterization
From 2009.igem.org
(Difference between revisions)
Line 190: | Line 190: | ||
var orgC = arsenicModelConstants(); | var orgC = arsenicModelConstants(); | ||
var cSol = {}; | var cSol = {}; | ||
- | for(var i in varsToMutate) | + | for(var i in varsToMutate) cSol[varsToMutate[i]] = 1; |
cSol.v5K5 = orgC.v5/orgC.K5; | cSol.v5K5 = orgC.v5/orgC.K5; | ||
cSol.K5 = orgC.K5; | cSol.K5 = orgC.K5; | ||
Line 220: | Line 220: | ||
var ENew = computeCost(cNew,experiments); | var ENew = computeCost(cNew,experiments); | ||
for(var a in cNew) { | for(var a in cNew) { | ||
- | + | var dca = (ENew-E)/(cNew[a]-c[a]); | |
- | + | ||
if (!(isNaN(dca) || !isFinite(dca))) dc[a] = (dc[a]+2*dca)/3; | if (!(isNaN(dca) || !isFinite(dca))) dc[a] = (dc[a]+2*dca)/3; | ||
} | } |
Revision as of 13:34, 30 September 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
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- Modelling
- DetailedModel
- Characterization
- Downloads
TODO: Talk about the devices we have and in what way we want to characterize them.
Uptake measurements
Time (min) | ||||||
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0 | 10 | 20 | 40 | 60 | ||
As(III)exT(0) (µM) | 0 | x | ||||
10 | x | x | x | x | x | |
20 | x | |||||
50 | x | |||||
100 | x |
To efficiently look at both time and concentration dependent processes we took samples as in the table on the right. Below we list all results, which have been used for fitting all necessary parameters.
TODO: List results. Take conversion from nmol/mg and mg/ml to µM and Vc/Vs into account.
best | cur | gradient | solved | |
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v5/K5 | ||||
v5 | ||||
K5 | ||||
k8/K7 | ||||
k8 | ||||
K7 | ||||
E |
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