Team:Virginia Commonwealth/Internal/Promoter characterization

From 2009.igem.org

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==Promoter Characterization Project Planning==
==Promoter Characterization Project Planning==
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*Table of BioBrick parts, concentrations and amounts
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*Table of triplets to be composed together
*Table of triplets to be composed together
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{| cellpadding="10" align="center" border="1" padding="5"
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|+
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|-
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! Promoter !! Gene (includes RBS and Terminator) !! Backbone !! E. Coli type
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|-
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!J23100
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| E0240 || pSB3K3 || NEB10β
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|-
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! J23101 
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| E0240 || pSB3K3 || NEB10β
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|-
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! J23102 
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| E0240 || pSB3K3 || NEB10β
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|-
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! J23103 
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| E0240 || pSB3K3 || NEB10β
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|-
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! J23104 
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| E0240 || pSB3K3 || NEB10β
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|-
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! J23105
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| E0240 || pSB3K3 || NEB10β
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|-
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! J23107
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| E0240 || pSB3K3 || NEB10β
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|-
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! J23110
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| E0240 || pSB3K3 || NEB10β
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|-
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! J23113
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| E0240 || pSB3K3 || NEB10β
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|-
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! J23116
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| E0240 || pSB3K3 || NEB10β
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|-
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! R0011
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| E0240 || pSB3K3 || NEB10β
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|-
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! R0040
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| E0240 || pSB3K3 || NEB10β
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|}
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*Table of BioBrick parts, concentrations and amounts
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 +
{| cellpadding="10" align="center" border="1" padding="5"
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|+
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|-
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! Part No. !! Concentration (microgram/milliliter) !! Amount (microgram)
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|-
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! J23100 
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| 14.0 || 5.32
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|-
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! J23103
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| 7.0 || 2.52
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|-
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! J23105 
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| 8.0 || 2.96
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|-
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! J23104 
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| 11.0 || 4.18
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|-
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! J23101 
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| 12.0 || 4.56
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|-
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! J23107
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| 18.0 || 8.1
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|-
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! J23116
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| 20.0 || 7.2
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|-
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! J23113
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| 14.0 || 5.18
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|-
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! J23110
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| 12.0 || 4.2
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|-
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! J23102
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| 31.0 || 10.23
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|-
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! R0011 
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| 21.0 || 5.46
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|-
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! R0040 
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| 15.0 || 5.4
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|-
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!pSB3K3 (Total)
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| n/a || 5.465
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|-
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!pSB3K3 (1)
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| 24 || .984
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|-
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!pSB3K3 (2)
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| 30 || 1.236
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|-
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!pSB3K3 (3)
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| 31.5 || 1.310
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|-
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!pSB3K3 (4)
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| 45 || 1.935
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|-
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!E0240 (Total)
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| n/a || 6.574
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|-
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!E0240 (1)
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| 30.0 || 1.200
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|-
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!E0240 (2)
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| 37.5 || 1.575
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|-
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!E0240 (3)
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| 45.0 || 1.868
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|-
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!E0240 (4)
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| 49.5 || 1.931
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|}
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 +
*

Revision as of 23:38, 15 June 2009

Promoter Characterization Project Planning

  • Table of triplets to be composed together
Promoter Gene (includes RBS and Terminator) Backbone E. Coli type
J23100 E0240 pSB3K3 NEB10β
J23101 E0240 pSB3K3 NEB10β
J23102 E0240 pSB3K3 NEB10β
J23103 E0240 pSB3K3 NEB10β
J23104 E0240 pSB3K3 NEB10β
J23105 E0240 pSB3K3 NEB10β
J23107 E0240 pSB3K3 NEB10β
J23110 E0240 pSB3K3 NEB10β
J23113 E0240 pSB3K3 NEB10β
J23116 E0240 pSB3K3 NEB10β
R0011 E0240 pSB3K3 NEB10β
R0040 E0240 pSB3K3 NEB10β
  • Table of BioBrick parts, concentrations and amounts
Part No. Concentration (microgram/milliliter) Amount (microgram)
J23100 14.0 5.32
J23103 7.0 2.52
J23105 8.0 2.96
J23104 11.0 4.18
J23101 12.0 4.56
J23107 18.0 8.1
J23116 20.0 7.2
J23113 14.0 5.18
J23110 12.0 4.2
J23102 31.0 10.23
R0011 21.0 5.46
R0040 15.0 5.4
pSB3K3 (Total) n/a 5.465
pSB3K3 (1) 24 .984
pSB3K3 (2) 30 1.236
pSB3K3 (3) 31.5 1.310
pSB3K3 (4) 45 1.935
E0240 (Total) n/a 6.574
E0240 (1) 30.0 1.200
E0240 (2) 37.5 1.575
E0240 (3) 45.0 1.868
E0240 (4) 49.5 1.931
Retrieved from "http://2009.igem.org/Team:Virginia_Commonwealth/Internal/Promoter_characterization"