Team:PKU Beijing/Notebook/AND Gate 1/Core/Rencheng Gao

From 2009.igem.org

(Difference between revisions)
(New page: {{PKU_Beijing/Header}} {{PKU_Beijing/Sidebar_Notebook}} {{PKU_Beijing/Header2}} ==='''2009.8.17'''=== '''0:50'''<br> Bacteria Culture: 11N-pcrS6(1~4), 2K-pcrS6(1~4), pla2+SupD(1~4), pcr2+...)
Line 7: Line 7:
Bacteria Culture: 11N-pcrS6(1~4), 2K-pcrS6(1~4), pla2+SupD(1~4), pcr2+SupD(1~4)
Bacteria Culture: 11N-pcrS6(1~4), 2K-pcrS6(1~4), pla2+SupD(1~4), pcr2+SupD(1~4)
-
'''12:00'''
+
'''12:00'''<br>
Miniprep: 2GpcrS6(1~4), 5JpcrS6(1~4), 1HpcrS6(1~4)
Miniprep: 2GpcrS6(1~4), 5JpcrS6(1~4), 1HpcrS6(1~4)
-
'''17:00'''
+
'''17:00'''<br>
-
Double-enzyme digestion assessment: 2GpcrS6(1~4), 5JpcrS6(1~4), 1HpcrS6(1~4)
+
Double-enzyme digestion assessment: 2GpcrS6(1~4), 5JpcrS6(1~4), 1HpcrS6(1~4)<br>
[[Image:PKU_20090817_Rencheng_Gao_1.JPG|400px]]
[[Image:PKU_20090817_Rencheng_Gao_1.JPG|400px]]
-
'''24:00'''
+
'''24:00'''<br>
Bacteria Culture: 1H-pcrS6(5~7), 11N-pcrS6(5~7), 2K-pcrS6(5~7), pla2+SupD(5~7), pcr2+SupD (5~7)
Bacteria Culture: 1H-pcrS6(5~7), 11N-pcrS6(5~7), 2K-pcrS6(5~7), pla2+SupD(5~7), pcr2+SupD (5~7)
==='''2009.8.18'''===
==='''2009.8.18'''===
-
'''11:00'''
+
'''11:00'''<br>
Miniprep: plaSupD(5~7), pcrSupD(5~7)
Miniprep: plaSupD(5~7), pcrSupD(5~7)
-
'''14:35'''
+
'''14:35'''<br>
-
Double-enzyme digestion assessment: plaSupD(5~7), pcrSupD(5~7)
+
Double-enzyme digestion assessment: plaSupD(5~7), pcrSupD(5~7)<br>
[[Image:PKU_20090818_Rencheng_Gao_1.JPG|400px]]
[[Image:PKU_20090818_Rencheng_Gao_1.JPG|400px]]
-
'''14:50'''
+
'''14:50'''<br>
-
Ligation:  
+
Ligation: <br>
-
Insert: 11N, 2K, 1H
+
Insert: 11N, 2K, 1H<br>
Vector: pcrS6
Vector: pcrS6
-
'''21:00'''
+
'''21:00'''<br>
Transformation
Transformation
==='''2009.8.19'''===
==='''2009.8.19'''===
-
'''15:00'''
+
'''15:00'''<br>
-
Double-enzyme digestion assessment
+
Double-enzyme digestion assessment<br>
Plasmid: plaS6, pcrS6, 5JpcrS6, 2GpcrS6
Plasmid: plaS6, pcrS6, 5JpcrS6, 2GpcrS6
-
'''22:00'''
+
'''22:00'''<br>
Bacteria culture: 2K(1~5), 1H(1~5)
Bacteria culture: 2K(1~5), 1H(1~5)
-
'''22:30'''
+
'''22:30'''<br>
Gel Purification: plaS6, pcrS6, 5JpcrS6, 2GpcrS6
Gel Purification: plaS6, pcrS6, 5JpcrS6, 2GpcrS6
-
'''00:30'''
+
'''00:30'''<br>
-
Ligation
+
Ligation<br>
-
Insert: plaS6, pcrS6, 5JpcrS6, 2GpcrS6
+
Insert: plaS6, pcrS6, 5JpcrS6, 2GpcrS6<br>
Vector: 1-7G
Vector: 1-7G
==='''2009.8.20'''===
==='''2009.8.20'''===
-
'''12:00'''
+
'''12:00'''<br>
Send samples for sequencing: plaS6, pcrS6, 5JpcrS6, 2GpcrS6
Send samples for sequencing: plaS6, pcrS6, 5JpcrS6, 2GpcrS6
-
'''14:00'''
+
'''14:00'''<br>
Miniprep: 2KpcrS6(1~5), 1HpcrS6(1~5), 11NpcrS6(1~5)
Miniprep: 2KpcrS6(1~5), 1HpcrS6(1~5), 11NpcrS6(1~5)
-
'''17:00'''
+
'''17:00'''<br>
Double-enzyme digestion: 2KpcrS6(1~5), 1HpcrS6(1~5), 11NpcrS6(1~5)
Double-enzyme digestion: 2KpcrS6(1~5), 1HpcrS6(1~5), 11NpcrS6(1~5)
-
'''22:00'''
+
'''22:00'''<br>
Store the strain: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)
Store the strain: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)
==='''2009.8.21'''===
==='''2009.8.21'''===
-
'''9:30'''
+
'''9:30'''<br>
Send samples for sequencing: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)
Send samples for sequencing: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)
==='''2009.8.22'''===
==='''2009.8.22'''===
-
'''11:00'''
+
'''11:00'''<br>
Bacteria culture: pcrS6(1~4), plaS6(1~4), 2GpcrS6(1~4), 5JpcrS6(1~4)
Bacteria culture: pcrS6(1~4), plaS6(1~4), 2GpcrS6(1~4), 5JpcrS6(1~4)
-
'''15:30'''
+
'''15:30'''<br>
-
Double-enzyme digestion: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)
+
Double-enzyme digestion: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)<br>
[[Image:PKU_20090822_Rencheng_Gao_1.JPG|400px]]
[[Image:PKU_20090822_Rencheng_Gao_1.JPG|400px]]
-
'''22:00'''
+
'''22:00'''<br>
Gel purification
Gel purification
-
'''23:00'''
+
'''23:00'''<br>
Miniprep: pcrS6(1~4), plaS6(1~4), 2GpcrS6(1~4), 5JpcrS6(1~4)
Miniprep: pcrS6(1~4), plaS6(1~4), 2GpcrS6(1~4), 5JpcrS6(1~4)
-
'''1:00'''
+
'''1:00'''<br>
-
Ligation:
+
Ligation:<br>
-
Insert: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)
+
Insert: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)<br>
Vector:1-7G
Vector:1-7G
==='''2009.8.23'''===
==='''2009.8.23'''===
-
'''13:00'''
+
'''13:00'''<br>
Double-enzyme digestion assessment: pcrS6(1~4), plaS6(1~4), 2GpcrS6(1~4), 5JpcrS6(1~4)
Double-enzyme digestion assessment: pcrS6(1~4), plaS6(1~4), 2GpcrS6(1~4), 5JpcrS6(1~4)
-
'''15:00'''
+
'''15:00'''<br>
Transformation
Transformation
-
'''0:00'''
+
'''0:00'''<br>
Bacteria culture: 5JpcrS6-low-copy, 2GpcrS6-low-copy
Bacteria culture: 5JpcrS6-low-copy, 2GpcrS6-low-copy
==='''2009.8.24'''===
==='''2009.8.24'''===
-
'''11:00'''
+
'''11:00'''<br>
Bacteria culture: 2KpcrS-low-copy(1~4), 1HpcrS-low-copy(1~4), 11NpcrS-low-copy(1~4)
Bacteria culture: 2KpcrS-low-copy(1~4), 1HpcrS-low-copy(1~4), 11NpcrS-low-copy(1~4)
-
'''14:00'''
+
'''14:00'''<br>
-
Double-enzyme digestion (back insert): T7pro-GFP
+
Double-enzyme digestion (back insert): T7pro-GFP<br>
Double-enzyme digestion (back vector): 5JpcrS6-low-copy, 2GpcrS6-low-copy
Double-enzyme digestion (back vector): 5JpcrS6-low-copy, 2GpcrS6-low-copy
-
'''21:00'''
+
'''21:00'''<br>
Gel purification
Gel purification
-
'''22:00'''
+
'''22:00'''<br>
Miniprep: 2KpcrS-low-copy(1~4), 1HpcrS-low-copy(1~4), 11NpcrS-low-copy(1~4)
Miniprep: 2KpcrS-low-copy(1~4), 1HpcrS-low-copy(1~4), 11NpcrS-low-copy(1~4)
-
'''23:30'''
+
'''23:30'''<br>
-
Ligation
+
Ligation<br>
-
Insert: T7pro-GFP
+
Insert: T7pro-GFP<br>
Vector: 5JpcrS6-low-copy, 2GpcrS6-low-copy
Vector: 5JpcrS6-low-copy, 2GpcrS6-low-copy
-
'''24:00'''
+
'''24:00'''<br>
Double-enzyme digestion: 2KpcrS-low-copy(1~4), 1HpcrS-low-copy(1~4), 11NpcrS-low-copy (1~4)
Double-enzyme digestion: 2KpcrS-low-copy(1~4), 1HpcrS-low-copy(1~4), 11NpcrS-low-copy (1~4)
==='''2009.8.25'''===
==='''2009.8.25'''===
-
'''13:30'''
+
'''13:30'''<br>
Transformation: 2G-T7pro-GFP, 5J-T7pro-GFP
Transformation: 2G-T7pro-GFP, 5J-T7pro-GFP
-
'''17:00'''
+
'''17:00'''<br>
Strain storage: 2KpcrS-low-copy, 1HpcrS-low-copy, 11NpcrS-low-copy
Strain storage: 2KpcrS-low-copy, 1HpcrS-low-copy, 11NpcrS-low-copy
==='''2009.8.27'''===
==='''2009.8.27'''===
-
'''14:30'''
+
'''14:30'''<br>
-
Inducement: AND gate(5J, 2G)
+
Inducement: AND gate(5J, 2G)<br>
-
HSL: 10-4M
+
HSL: 10-4M<br>
Arabinose: 10-4M
Arabinose: 10-4M
-
'''17:00'''
+
'''17:00'''<br>
Double-enzyme digestion (front insert): 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)
Double-enzyme digestion (front insert): 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)
==='''2009.8.28'''===
==='''2009.8.28'''===
-
'''13:00'''
+
'''13:00'''<br>
-
Inducement: AND gate(5J, 2G)
+
Inducement: AND gate(5J, 2G)<br>
-
HSL: 10-4M
+
HSL: 10-4M<br>
Arabinose: 10-4M
Arabinose: 10-4M
-
'''13:40'''
+
'''13:40'''<br>
Double-enzyme digestion (front insert): T7pro-GFP
Double-enzyme digestion (front insert): T7pro-GFP
==='''2009.8.29'''===
==='''2009.8.29'''===
-
'''13:00'''
+
'''13:00'''<br>
Bacteria culture: 1HpcrS(1~3), 11NpcrS(1~3), 2KpcrS(1~3), 5JpcrS(1~3), 2GpcrS(1~3)
Bacteria culture: 1HpcrS(1~3), 11NpcrS(1~3), 2KpcrS(1~3), 5JpcrS(1~3), 2GpcrS(1~3)
-
'''21:45'''
+
'''21:45'''<br>
-
Ligation
+
Ligation<br>
-
Insert: 5NT7ptag, 2MT7ptag, 2IT7ptag, 1JT7ptag
+
Insert: 5NT7ptag, 2MT7ptag, 2IT7ptag, 1JT7ptag<br>
Vector: pcrS6
Vector: pcrS6
==='''2009.8.30'''===
==='''2009.8.30'''===
-
'''13:00'''
+
'''13:00'''<br>
Miniprep: 1HpcrS(1~3), 11NpcrS(1~3), 2KpcrS(1~3), 5JpcrS(1~3), 2GpcrS(1~3)
Miniprep: 1HpcrS(1~3), 11NpcrS(1~3), 2KpcrS(1~3), 5JpcrS(1~3), 2GpcrS(1~3)
-
'''14:00'''
+
'''14:00'''<br>
Double-enzyme digestion assessment: 1HpcrS(1~3), 11NpcrS(1~3), 2KpcrS(1~3), 5JpcrS(1~3), 2GpcrS(1~3)
Double-enzyme digestion assessment: 1HpcrS(1~3), 11NpcrS(1~3), 2KpcrS(1~3), 5JpcrS(1~3), 2GpcrS(1~3)
-
'''17:00'''
+
'''17:00'''<br>
Bacteria culture: 5NT7ptag(1~3), 2MT7ptag(1~3), 2IT7ptag(1~3), 1JT7ptag(1~3)
Bacteria culture: 5NT7ptag(1~3), 2MT7ptag(1~3), 2IT7ptag(1~3), 1JT7ptag(1~3)
-
'''21:30'''
+
'''21:30'''<br>
Strain Storage: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)
Strain Storage: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)
-
'''22:00'''
+
'''22:00'''<br>
Double-enzyme digestion (front vector): 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)
Double-enzyme digestion (front vector): 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)
==='''2009.8.31'''===
==='''2009.8.31'''===
-
'''13:00'''
+
'''13:00'''<br>
Miniprep: 5NT7ptag(1~3), 2MT7ptag(1~3), 2IT7ptag(1~3), 1JT7ptag(1~3)
Miniprep: 5NT7ptag(1~3), 2MT7ptag(1~3), 2IT7ptag(1~3), 1JT7ptag(1~3)
-
'''14:00'''
+
'''14:00'''<br>
Gel purification: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)
Gel purification: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)
-
'''16:00'''
+
'''16:00'''<br>
Send samples for sequencing: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)
Send samples for sequencing: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)
-
'''17:00'''
+
'''17:00'''<br>
-
Ligation:
+
Ligation:<br>
Vector: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)
Vector: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)
-
'''17:30'''
+
'''17:30'''<br>
Double-enzyme digestion: 5NT7ptag(1~3), 2MT7ptag(1~3), 2IT7ptag(1~3), 1JT7ptag(1~3)
Double-enzyme digestion: 5NT7ptag(1~3), 2MT7ptag(1~3), 2IT7ptag(1~3), 1JT7ptag(1~3)
-
'''1:30'''
+
'''1:30'''<br>
Strain Storage: 2I(1), 2M(2), 1J(2), 5N(1)
Strain Storage: 2I(1), 2M(2), 1J(2), 5N(1)
==='''2009.9.1'''===
==='''2009.9.1'''===
-
'''13:30'''
+
'''13:30'''<br>
-
Double-enzyme digestion (Front Vector)
+
Double-enzyme digestion (Front Vector)<br>
Plasmid: 5N, 2M, 2I, 1J
Plasmid: 5N, 2M, 2I, 1J
-
'''21:30'''
+
'''21:30'''<br>
Culture the bacteria: 5J, 2K, 1H, 2G-T7pro GFP
Culture the bacteria: 5J, 2K, 1H, 2G-T7pro GFP
-
'''23:20'''
+
'''23:20'''<br>
-
Ligation
+
Ligation<br>
-
Vector: 5N, 2M, 2I 2J
+
Vector: 5N, 2M, 2I 2J<br>
Insert: T7pro-GFP
Insert: T7pro-GFP
==='''2009.9.2'''===
==='''2009.9.2'''===
-
'''14:00'''
+
'''14:00'''<br>
Inducement: 5J, 2K, 1H, 2G, 11N
Inducement: 5J, 2K, 1H, 2G, 11N
-
'''22:00'''
+
'''22:00'''<br>
-
Double-enzyme digestion
+
Double-enzyme digestion<br>
Plasmid: 1JpcrS, 11NpcrS, 2IpcrS, 5JpcrS, 2MpcrS, 1HpcrS, 5NpcrS, 2GpcrS, 2KpcrS
Plasmid: 1JpcrS, 11NpcrS, 2IpcrS, 5JpcrS, 2MpcrS, 1HpcrS, 5NpcrS, 2GpcrS, 2KpcrS
==='''2009.9.4'''===
==='''2009.9.4'''===
-
'''15:30'''
+
'''15:30'''<br>
Send samples for sequencing: 5N, 2M, 2I, 1J
Send samples for sequencing: 5N, 2M, 2I, 1J
-
'''15:45'''
+
'''15:45'''<br>
Gel purification: 11N, 1H, 2M, 1J, 2G, 5N, 1J
Gel purification: 11N, 1H, 2M, 1J, 2G, 5N, 1J
-
'''16:00'''
+
'''16:00'''<br>
-
Ligation:
+
Ligation:<br>
-
Vector: 1-7G
+
Vector: 1-7G<br>
Insert: 11N, 1H, 2M, 1J, 2G, 5N, 1J
Insert: 11N, 1H, 2M, 1J, 2G, 5N, 1J
==='''2009.9.5'''===
==='''2009.9.5'''===
-
'''17:00'''
+
'''17:00'''<br>
-
Double-enzyme digestion
+
Double-enzyme digestion<br>
Plasmid: 11N(1), 2M(2), 2K(2), 1H(1), 5J(1), 1J(2), 2G(2)
Plasmid: 11N(1), 2M(2), 2K(2), 1H(1), 5J(1), 1J(2), 2G(2)
==='''2009.9.6'''===
==='''2009.9.6'''===
-
'''16:00'''
+
'''16:00'''<br>
Double-enzyme digestion (5J, 2I, 1J, 2K, 1H, 5N, 2M, 11N, 2G)
Double-enzyme digestion (5J, 2I, 1J, 2K, 1H, 5N, 2M, 11N, 2G)
-
'''22:00'''
+
'''22:00'''<br>
Culture the bacteria: 5N(3), 2I(1), 2I(3)
Culture the bacteria: 5N(3), 2I(1), 2I(3)
-
'''00:00'''
+
'''00:00'''<br>
-
Ligation:
+
Ligation:<br>
-
Insert: 5J, 2K, 1H, 2M, 2G
+
Insert: 5J, 2K, 1H, 2M, 2G<br>
Vector: 1-7G
Vector: 1-7G
==='''2009.9.7'''===
==='''2009.9.7'''===
-
'''14:00'''
+
'''14:00'''<br>
Mini-prep: 2I(1), 5N(3), 2I(3)
Mini-prep: 2I(1), 5N(3), 2I(3)
-
'''16:30'''
+
'''16:30'''<br>
Double-enzyme digestion: 1J
Double-enzyme digestion: 1J
-
'''17:00'''
+
'''17:00'''<br>
Prepare competent cells
Prepare competent cells
-
'''21:00'''
+
'''21:00'''<br>
Transformation: 2G, 2I(1), 2I(3), 5N(3)
Transformation: 2G, 2I(1), 2I(3), 5N(3)
==='''2009.9.8'''===
==='''2009.9.8'''===
-
'''14:00'''
+
'''14:00'''<br>
Culture the bacteria:
Culture the bacteria:
-
'''15:00'''
+
'''15:00'''<br>
-
Ligation
+
Ligation<br>
-
Insert: 2M, 1H
+
Insert: 2M, 1H<br>
Vector: 1-7G
Vector: 1-7G
==='''2009.9.9'''===
==='''2009.9.9'''===
-
'''01:00'''
+
'''01:00'''<br>
-
Double-enzyme digestion
+
Double-enzyme digestion<br>
Plasmid: 11N, 5J, 2K
Plasmid: 11N, 5J, 2K
-
'''20:30'''
+
'''20:30'''<br>
Inducement: 2G, 5N, 2I
Inducement: 2G, 5N, 2I
-
'''24:00'''
+
'''24:00'''<br>
Mini-prep: 1J(1-3), 2M(1-3), 1H(1-3)
Mini-prep: 1J(1-3), 2M(1-3), 1H(1-3)
{{PKU_Beijing/Foot}}
{{PKU_Beijing/Foot}}
__NOTOC__
__NOTOC__

Revision as of 14:13, 1 October 2009
 

2009.8.17

0:50
Bacteria Culture: 11N-pcrS6(1~4), 2K-pcrS6(1~4), pla2+SupD(1~4), pcr2+SupD(1~4)

12:00
Miniprep: 2GpcrS6(1~4), 5JpcrS6(1~4), 1HpcrS6(1~4)

17:00
Double-enzyme digestion assessment: 2GpcrS6(1~4), 5JpcrS6(1~4), 1HpcrS6(1~4)
PKU 20090817 Rencheng Gao 1.JPG

24:00
Bacteria Culture: 1H-pcrS6(5~7), 11N-pcrS6(5~7), 2K-pcrS6(5~7), pla2+SupD(5~7), pcr2+SupD (5~7)

2009.8.18

11:00
Miniprep: plaSupD(5~7), pcrSupD(5~7)

14:35
Double-enzyme digestion assessment: plaSupD(5~7), pcrSupD(5~7)
PKU 20090818 Rencheng Gao 1.JPG

14:50
Ligation:
Insert: 11N, 2K, 1H
Vector: pcrS6

21:00
Transformation

2009.8.19

15:00
Double-enzyme digestion assessment
Plasmid: plaS6, pcrS6, 5JpcrS6, 2GpcrS6

22:00
Bacteria culture: 2K(1~5), 1H(1~5)

22:30
Gel Purification: plaS6, pcrS6, 5JpcrS6, 2GpcrS6

00:30
Ligation
Insert: plaS6, pcrS6, 5JpcrS6, 2GpcrS6
Vector: 1-7G

2009.8.20

12:00
Send samples for sequencing: plaS6, pcrS6, 5JpcrS6, 2GpcrS6

14:00
Miniprep: 2KpcrS6(1~5), 1HpcrS6(1~5), 11NpcrS6(1~5)

17:00
Double-enzyme digestion: 2KpcrS6(1~5), 1HpcrS6(1~5), 11NpcrS6(1~5)

22:00
Store the strain: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)

2009.8.21

9:30
Send samples for sequencing: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)

2009.8.22

11:00
Bacteria culture: pcrS6(1~4), plaS6(1~4), 2GpcrS6(1~4), 5JpcrS6(1~4)

15:30
Double-enzyme digestion: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)
PKU 20090822 Rencheng Gao 1.JPG

22:00
Gel purification

23:00
Miniprep: pcrS6(1~4), plaS6(1~4), 2GpcrS6(1~4), 5JpcrS6(1~4)

1:00
Ligation:
Insert: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)
Vector:1-7G

2009.8.23

13:00
Double-enzyme digestion assessment: pcrS6(1~4), plaS6(1~4), 2GpcrS6(1~4), 5JpcrS6(1~4)

15:00
Transformation

0:00
Bacteria culture: 5JpcrS6-low-copy, 2GpcrS6-low-copy

2009.8.24

11:00
Bacteria culture: 2KpcrS-low-copy(1~4), 1HpcrS-low-copy(1~4), 11NpcrS-low-copy(1~4)

14:00
Double-enzyme digestion (back insert): T7pro-GFP
Double-enzyme digestion (back vector): 5JpcrS6-low-copy, 2GpcrS6-low-copy

21:00
Gel purification

22:00
Miniprep: 2KpcrS-low-copy(1~4), 1HpcrS-low-copy(1~4), 11NpcrS-low-copy(1~4)

23:30
Ligation
Insert: T7pro-GFP
Vector: 5JpcrS6-low-copy, 2GpcrS6-low-copy

24:00
Double-enzyme digestion: 2KpcrS-low-copy(1~4), 1HpcrS-low-copy(1~4), 11NpcrS-low-copy (1~4)

2009.8.25

13:30
Transformation: 2G-T7pro-GFP, 5J-T7pro-GFP

17:00
Strain storage: 2KpcrS-low-copy, 1HpcrS-low-copy, 11NpcrS-low-copy

2009.8.27

14:30
Inducement: AND gate(5J, 2G)
HSL: 10-4M
Arabinose: 10-4M

17:00
Double-enzyme digestion (front insert): 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)

2009.8.28

13:00
Inducement: AND gate(5J, 2G)
HSL: 10-4M
Arabinose: 10-4M

13:40
Double-enzyme digestion (front insert): T7pro-GFP

2009.8.29

13:00
Bacteria culture: 1HpcrS(1~3), 11NpcrS(1~3), 2KpcrS(1~3), 5JpcrS(1~3), 2GpcrS(1~3)

21:45
Ligation
Insert: 5NT7ptag, 2MT7ptag, 2IT7ptag, 1JT7ptag
Vector: pcrS6

2009.8.30

13:00
Miniprep: 1HpcrS(1~3), 11NpcrS(1~3), 2KpcrS(1~3), 5JpcrS(1~3), 2GpcrS(1~3)

14:00
Double-enzyme digestion assessment: 1HpcrS(1~3), 11NpcrS(1~3), 2KpcrS(1~3), 5JpcrS(1~3), 2GpcrS(1~3)

17:00
Bacteria culture: 5NT7ptag(1~3), 2MT7ptag(1~3), 2IT7ptag(1~3), 1JT7ptag(1~3)

21:30
Strain Storage: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)

22:00
Double-enzyme digestion (front vector): 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)

2009.8.31

13:00
Miniprep: 5NT7ptag(1~3), 2MT7ptag(1~3), 2IT7ptag(1~3), 1JT7ptag(1~3)

14:00
Gel purification: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)

16:00
Send samples for sequencing: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)

17:00
Ligation:
Vector: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)

17:30
Double-enzyme digestion: 5NT7ptag(1~3), 2MT7ptag(1~3), 2IT7ptag(1~3), 1JT7ptag(1~3)

1:30
Strain Storage: 2I(1), 2M(2), 1J(2), 5N(1)

2009.9.1

13:30
Double-enzyme digestion (Front Vector)
Plasmid: 5N, 2M, 2I, 1J

21:30
Culture the bacteria: 5J, 2K, 1H, 2G-T7pro GFP

23:20
Ligation
Vector: 5N, 2M, 2I 2J
Insert: T7pro-GFP

2009.9.2

14:00
Inducement: 5J, 2K, 1H, 2G, 11N

22:00
Double-enzyme digestion
Plasmid: 1JpcrS, 11NpcrS, 2IpcrS, 5JpcrS, 2MpcrS, 1HpcrS, 5NpcrS, 2GpcrS, 2KpcrS

2009.9.4

15:30
Send samples for sequencing: 5N, 2M, 2I, 1J

15:45
Gel purification: 11N, 1H, 2M, 1J, 2G, 5N, 1J

16:00
Ligation:
Vector: 1-7G
Insert: 11N, 1H, 2M, 1J, 2G, 5N, 1J

2009.9.5

17:00
Double-enzyme digestion
Plasmid: 11N(1), 2M(2), 2K(2), 1H(1), 5J(1), 1J(2), 2G(2)

2009.9.6

16:00
Double-enzyme digestion (5J, 2I, 1J, 2K, 1H, 5N, 2M, 11N, 2G)

22:00
Culture the bacteria: 5N(3), 2I(1), 2I(3)

00:00
Ligation:
Insert: 5J, 2K, 1H, 2M, 2G
Vector: 1-7G

2009.9.7

14:00
Mini-prep: 2I(1), 5N(3), 2I(3)

16:30
Double-enzyme digestion: 1J

17:00
Prepare competent cells

21:00
Transformation: 2G, 2I(1), 2I(3), 5N(3)

2009.9.8

14:00
Culture the bacteria:

15:00
Ligation
Insert: 2M, 1H
Vector: 1-7G

2009.9.9

01:00
Double-enzyme digestion
Plasmid: 11N, 5J, 2K

20:30
Inducement: 2G, 5N, 2I

24:00
Mini-prep: 1J(1-3), 2M(1-3), 1H(1-3)

^Top


 

Retrieved from "http://2009.igem.org/Team:PKU_Beijing/Notebook/AND_Gate_1/Core/Rencheng_Gao"