Team:Newcastle/Labwork/15 September 2009

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==<u>Stochastic Switch Team</u>==
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Today we set up another restriction disest of the KinA pMKRQ synthesised brick using NheI and EcoRI we ran this on an 0.8% gel however we think that this may be too dilute and that I why we are not seeing the two bands ogf the digest which are a similar size.
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We also set up another PCR of the ara fragment  as a back up plan for ligation, in case the last ligation did not work as well as we had planned.
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We also mini prepped and digested the sac transformations again, and we found that of the 6 samples we had, a few of them had worked!
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Just to confirm however we set up 12 more miniprep cultures again for both sac and ara.
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Yesterday we did midipreps of Goksels successful sspb plasmid: this was cut with XbaI and PstI ready for ligation into the ara brick (once it's ligated!).
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Revision as of 20:33, 1 October 2009

Lab Work - 15/09/09

Stochastic Switch Team

Today we set up another restriction disest of the KinA pMKRQ synthesised brick using NheI and EcoRI we ran this on an 0.8% gel however we think that this may be too dilute and that I why we are not seeing the two bands ogf the digest which are a similar size.

We also set up another PCR of the ara fragment as a back up plan for ligation, in case the last ligation did not work as well as we had planned.

We also mini prepped and digested the sac transformations again, and we found that of the 6 samples we had, a few of them had worked!

Just to confirm however we set up 12 more miniprep cultures again for both sac and ara.

Yesterday we did midipreps of Goksels successful sspb plasmid: this was cut with XbaI and PstI ready for ligation into the ara brick (once it's ligated!).



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