Revision as of 22:53, 1 October 2009

Meetings Calendar

Lab Work - 17/09/09

Stochastic switch team

Today we ran the minipreps for sac and ara transformed cells however- perhaps due to poor miniprep procedure- the sac test digest yeilded poor results, however the ara transformants miniprep gave a ~200bp band along with the backbone in 8/12 lanes:

Midi prep cultures were set up using cultures corresponding to lanes 1 and 3 for ara.

To test whether the poor sac result was due to the miniprep more miniprep cultures were set up to do tomorrow.

Today also we did fragment preps for Goksels sspb the ara fragment, and a cwlJ fragment for Hanny:

20 – 21 June 2009 - Europe workshop (London)
23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
23 October Practice Presentation (Newcastle)
23 October T-shirts are ready
27 October Practice Presentation (Sunderland)
27 October Poster is ready
30 October – 2 November 2009 - Jamboree (Boston)

Newcastle iGEM Twitter
[http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
[http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]

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 __NOTOC__
 =Lab Work - 17/09/09=
+=Stochastic switch team=
+Today we ran the minipreps for sac and ara transformed cells however- perhaps due to poor miniprep procedure- the sac test digest yeilded poor results, however the ara transformants miniprep gave a ~200bp band along with the backbone in 8/12 lanes: [[Image:Team NewcastleAra 170909.png]]
+Midi prep cultures were set up using cultures corresponding to lanes 1 and 3 for ara.
+To test whether the poor sac result was due to the miniprep more miniprep cultures were set up to do tomorrow.
+Today also we did fragment preps for Goksels sspb the ara fragment, and a cwlJ fragment for Hanny: [[Image:Team NewcFragpreps1.png]]
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Team:Newcastle/Labwork/17 September 2009

From 2009.igem.org

Revision as of 22:53, 1 October 2009

Lab Work - 17/09/09

Stochastic switch team