Team:PKU Beijing/Notebook/AND Gate 1/Input/TetR 2

From 2009.igem.org

(Difference between revisions)
Lug7 (Talk | contribs)
(New page: {{PKU_Beijing/Header}} {{PKU_Beijing/Sidebar_Notebook}} {{PKU_Beijing/Header2}} ==='''2009.8.16'''=== Test if the tetR promoter system works well in the low-copy backbone.<br> Get tetR+pr...)
Newer edit →

Revision as of 10:55, 2 October 2009
 

2009.8.16

Test if the tetR promoter system works well in the low-copy backbone.
Get tetR+promoter+GRP from Wu Shuke.

16:40
Digest the promoter and reporter system.

Total50μL
Plasmids5μL
EcoR11μL
Pst11μL
Buffer2μL
ddH2O11μL

20:00
Electrophoresis to recycle the inserts.
The order of the samples: marker, digestion products, plasmids control.
Results:
PKU 20090816 Shan Shen 1.JPG
Only the first one is correctly digested, recycle them.

2009.8.17

10:00
Link the inserts with vectors with has Kanamycin resistance.

17:50
Transformation.

19:30
Start to incubate.

2009.8.18

10:00
There are many colonies on the plate.
PCR colonies to test if they are correct.

^Top


 

Retrieved from "http://2009.igem.org/Team:PKU_Beijing/Notebook/AND_Gate_1/Input/TetR_2"