Team:Newcastle/Labwork/14 September 2009

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===Practical Outline===
===Practical Outline===
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Today's lab session will continue straight on from yesterday's session and fulfil the following objectives:
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Today's lab session will continue straight on from the last lab session and fulfil the following objectives:
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<br>
* Run products of PCR reaction involving the arsR BioBrick (BBa_J33206 in pSB1A2 missing promoter) and cadA promoter region  
* Run products of PCR reaction involving the arsR BioBrick (BBa_J33206 in pSB1A2 missing promoter) and cadA promoter region  
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** Ligate the fragments (possibly overnight)  
** Ligate the fragments (possibly overnight)  
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===Procedure===
===Procedure===
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Revision as of 15:06, 5 October 2009

Lab Work - 14/09/09

Metal Sensing team

Introduction

Practical Outline

Today's lab session will continue straight on from the last lab session and fulfil the following objectives:

  • Run products of PCR reaction involving the arsR BioBrick (BBa_J33206 in pSB1A2 missing promoter) and cadA promoter region
    • If successful clean up product, run on agarose gel through electrophoresis, excise band and carry out gel extraction.
    • Cut cleaned and excised fragment with BamHI and NheI
    • Carry out ligation (possibly an overnight ligation)
  • Cut pMUTIN4 and cotC (i.e. PCR product 3) with BamHI and HindIII
    • Ligate the fragments (possibly overnight)


Procedure



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