Team:Newcastle/Labwork/24 September 2009

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=Lab Work - 24/09/09=
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=Formal Lab Session - 24th September 2009=
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=Stochastic switch team=

Revision as of 16:19, 6 October 2009

Team Newcastle 2009 iGEM ProbationaryP-Sign.PNG

Formal Lab Session - 24th September 2009

Stochastic switch team

Today we did minipreps of the ara/sspb/pSB1AT3 cultures which were then digested with EcoRI and SpeI. These were run on an 0.8% gel. We expected to see a ~600bp band as well as the plasmid backbone and the gel photograph seemed fairly convincing:

Team Newcastle240909 trans mini.png

We set up a 50ml midiprep culture from the culture corresponding to lane 9 which seemed to have the clearest band.




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