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Keep track with what we did so far

(09.10.09)

This week we have done the following :

• We did a characterization assay with different time of exposure to the light
• We did a characterization assay with 2 hours of exposure to light, with the plate reader and the qPCR machine
• Digestion assay with the potential double transformant in the Trp K.O. strains

(02.10.09)

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(25.09.09)

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(18.09.09)

This week we have done less wet lab, as the academical year has begun (so we had to go to our courses):

(11.09.09)

This tenth week of wetlab we have done the following things:

• RO1 has been characterized

(04.09.09)

This ninth week of wetlab we have done the following things:

• RO2 has been characterized

(29.08.09)

This eighth week of wetlab we have done the following things:

• Cultures to clone the biobrick in front of RO2 -> didn't work
• Double transformation
• Send to sequencing
• RO1 is yet not working

(22.08.09)

This seventh week of wetlab we have done the following things:

• Beginning of the RbphP project (PCR colony)
• Try of 1.5 step PCR to get the complete biobrick from LovTap -> didn't work
• Check (digestion assay) to confirm we have the LovTap-term
• Try of medium M9 for RO2
• Characterisation (fluorescence in fonction of induction) for RO2
• From LovTap-term, ligation to obtain the biobrick LacI-Rbs-LovTap-term with cloning steps (not from the 1.5 step PCR)

(15.08.09)

This sixth week of wetlab we have done the following things:

• Protocol of ligation was refined.
• !!! Inducible LOVTAP-term and Read out 2 biobrick were created.!!! The system is completed.
• They need to be sequenced, characterized and submitted to the registery.

(08.08.09)

This fifth week of wetlab we have done the following things:

• Problem in ligations.

(01.08.09)

This fourth week of wetlab we have done the following things:

• Our gene of interest (photoreceptor LOVTAP) was cloned in front of a terminator (iGEM part [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]). We created our first biobrick.
• The second biobrick was coloned as well, but we wait for the results to confirm that the plasmid contain the right insert
• Modeling part: Preliminar simulations were lauched, and their analysis were done. Simulations will be lauched in few days.

(24.07.09)

This third week of wetlab we have done the following things:

• Again : Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
• Modeling part: Preliminar simulation was launched over the weekend.

(17.07.09)

This second week of wetlab we have done the following things:

• Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
• Modeling part: Files required to launch simulations were created and analyzed.

(12.07.09)

This first week of wetlab we have done the following things:

• Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
• Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
• Ordered and received the primers needed for the PCR of LovTAP
• Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
• Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
• Fused the two BioBricks "LacI" and "RBS"
• Digested the LovTAP PCR products and RBS part