Team:UNIPV-Pavia/Notebook/Week2Oct

From 2009.igem.org

(Difference between revisions)
m (October, 9th)
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== <html><font class="dayw_style">October, 9th</font></html> ==
== <html><font class="dayw_style">October, 9th</font></html> ==
-
5th experiment - with this experiment we try to give E.coli a pH and sodium shock in order to see if something happens.
+
5th experiment (with this experiment we try to give E.coli a pH and sodium shock in order to see if something happens).
-
*We put 50 ul into 5 ml LB NaCl 70 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four five hours.
+
*We put 50 ul into 5 ml LB NaCl 70 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four four hours and a half.
*OD measure of the three falcon with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
*OD measure of the three falcon with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
*We diluted:
*We diluted:

Revision as of 13:15, 11 October 2009

EthanolPVanimation.gif

December 2008
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March 2009
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April 2009
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May 2009
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June 2009
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July 2009
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August 2009
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September 2009
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October 2009
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November 2009
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30

Week from October 5th, to October 11st, 2009

Previous Week Next Week

October, 5th

pH sensor
  • Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
    • RBS33
    • A2
    • pNhaA
  • Overnight incubation of the three cultures.

Top

October, 6th

4th experiment

  • We put 50 ul into 5 ml LB NaCl 250 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four five hours.
  • OD measure of the three falcon with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
  • We diluted:
    • RBS into:
      • LB NaCl 250 mM + Amp pH 5,5
      • LB NaCl 250 mM + Amp pH 6,6
      • LB NaCl 250 mM + Amp pH 7,5
      • LB NaCl 250 mM + Amp pH 8,5
    • pNhaA into:
      • LB NaCl 250 mM + Amp pH 5,5
      • LB NaCl 250 mM + Amp pH 6,6
      • LB NaCl 250 mM + Amp pH 7,5
      • LB NaCl 250 mM + Amp pH 8,5
    • A2 into:
      • LB NaCl 250 mM + Amp pH 5,5
      • LB NaCl 250 mM + Amp pH 6,6
      • LB NaCl 250 mM + Amp pH 7,5
      • LB NaCl 250 mM + Amp pH 8,5
  • We started this 24 hours experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.

Top

October, 7th

pH sensor

Qui risultati?


Top

October, 8th

pH sensor
  • Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
    • RBS33
    • A2
    • pNhaA
  • Overnight incubation of the three cultures.


Top

October, 9th

5th experiment (with this experiment we try to give E.coli a pH and sodium shock in order to see if something happens).

  • We put 50 ul into 5 ml LB NaCl 70 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four four hours and a half.
  • OD measure of the three falcon with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
  • We diluted:
    • RBS into:
      • LB NaCl 600 mM + Amp pH 10
      • LB NaCl 600 mM + Amp pH 11,2
    • pNhaA into:
      • LB NaCl 600 mM + Amp pH 10
      • LB NaCl 600 mM + Amp pH 11,2
    • A2 into:
      • LB NaCl 600 mM + Amp pH 10
      • LB NaCl 600 mM + Amp pH 11,2
  • We started this 6 hours experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.


Top

October, 10th

Top

October, 11th

Top


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