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Team:KU Seoul/Notebook
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==Lab== | ==Lab== | ||
Revision as of 13:14, 12 October 2009
Contents |
Lab
Protocols (from elsewhere and FAQs)
- How do I test the transformation efficiency of my competent cells?
- Please see the protocol on how to [http://www.openwetware.org/wiki/TOP10_chemically_competent_cells make competent cells] for instructions on how to calculate transformation efficiency.
Discussion
Team building
From instructors
- 10.07.09
We had a review on our two ideas from Prof.Choi. He told us the ideas are creative and good but not fit for IGEM's concept. We need more feedback and information on our ideas. We need to get more familiar with IGEM's tools. Two additional ideas about Scromboid food poisoning and lactic acid degradation were referred at the end.
- 17.07.09
Till our next meeting, we should study how systemic biology works and understand related tools. Cumulate ideas. In other words, brainstorm consistently. Hyuk-jin will help with setting up experiments. Lab note opend.
- 24.07.09
We narrowed our ideas in to two. They are "Metal Detector" and "Bactaeria Compass." Think about what parts would be used and how you would build the circuits. Visit the former iGEM teams and study how they carried on their projects.
- 31.07.09
Find out the exact mechanism of metal detection. Look up the parts that are available on iGEM home. See how various metals can be detected by using E.coli.
- 07.08.09
We decided to detect 4 metals; Cd, Hg, Pb, and Ars. They are all critical to the health if ingested. Think about what the outcome would be after detection. Because there are 4 metals, you can bring out 16 different results by combining them. It seems that the most vialbe instrument would be light.
- 14.08.09
Study what parts on the registry we can use and how you would build a circuit. Increase your knowdlege on circutis by studying former iGEM teams. Remember, imitation is the mother of creation.
- 21.08.09
Vacation is almost over. The parts that we have looked for are mostly not available in the iGEM 2009 distribution registry, except for lead. We might have to construct the parts ourselves. Once we have our parts and our circuit models, doing experiments is a piece of cake. It's time to fuel up. Look for sponsors.
Brainstroming
Ideas
- Biosurfactants
- Keratinase
- Deodorant
- Perfume-producing bacteria
- Heat absorber
- Bio pencil
Should we focus on the result, or the procedure?
Decision making
From Ingeol Choi, 15th of August
1. You can sense and bind mercury with MerR ( However, it might bind other metals as well.)
2. It would be great if we bind all four different metals simultaneously( which should be hard).
3. Talk with Hyukjin for experiment planning.
4. Synthetic Biology team is going on a trip for three days. We won't be able to make it on Friday meeting.
From Simin Kim, 14th of August
IGEM 2007 team has already tried mercury metal sensor, but they failed to make it work. (http://parts.mit.edu/igem07/index.php/MIT) I think this is due to lack of appropriate biobricks registered. I am looking through other teams to study. Hope we can all meet on Friday. See you all.
From Ingeol Choi, 15th of August
1. Signal in: Mercury detection: MerR - mercury binding protein regulator
2. Signal out: amd gene cloned by Hyukjin (Able to detect with Tyrenol)
References
http://mic.sgmjournals.org/cgi/content/abstract/152/3/709
http://www.ncbi.nlm.nih.gov/pubmed/10503547
http://www.sciencemag.org/cgi/content/abstract/301/5638/1383 (copper?)
From Youngseol Byun, 14th of August
I designed our mascot as I mentioned. I will put it up on wiki. See how it looks.
From Ingeol Choi, 13th of August
https://2009.igem.org/Judging has been posted. See what is needed. Confirm who's going to the Jamboree.
From Cheolwon Choi, 8th of August
I finished our brocheure. Let's make a list of places to visit for our fund raising.
From Hansung Roh, 8th of August
Participants : Youngseol Byun, Hansung Roh, Hyukjin Ko, Cheolwoo Lee
Discussion 1 : We need to study the mechanisms for our parts. Understand how they work.
Discussion 2 : We are going to have our detector show the difference in concentration. Check out the former iGEM team that worked on Vanillin.
Conclusion : Study circuits and how to build them.
From Ingeol Choi, 7th of August
Jamboree registration starts at last. Get excited!!!
From Young Byun, 7th of August
How is creating our mascot a bacteria holding a metal detctor? I will work on it.
From Cheolwon Choi, 6th of August
Thanks for the feedbacks. Can you guys send me your thoughts about iGEM to put them in our brocheure. Slogans for the brocheure are also welcome.
From Hansung Roh, 6th of August
We need a decent title for the brocheure. How's 'We design life!!! - Metal detecting cell' ? We need something strong!
From Cheolwon Choi, 5th of August
I agree with Young.
I made a brocheure for our fund raising. I need feedback.
From Young Byun, 5th of August
I think the logo should contain something to do with our project. Nice work, though.
From Yoonji Kim, 5th of August
I made our logo for iGEM. They come in two different colors. See and how you like it. Give me suggestions.
From Ingeol Choi, 3rd of August
Don't worry. Team Gronigen's project and ours is quite different. Update our project discription for wiki.
From Cheolwon Choi, 4th of August
Thanks everyone for your participation inspite of vacation. I refined my ideas and made some fixes with the diagram I sent before. See what changed.
From Young Byun, 4th of August
Team Gronigen seems to do something similar with us. https://2009.igem.org/Team:Groningen Check it out.
From Young Byun, 3rd of August
Among the teams of iGEM 2007, there's a device that compares A and B's concentration. I think we can use this to figure out which metal is contained more.
I have looked up the parts for Lead, Cadmium, Mercury as Cheolwon had suggested. As for lead, it is confirmed that it works well but for cadmium and mercury we have to try them ourselves.
I added an excel file that shows the parts I looked up.
From Hansung Roh, 3rd of August
I looked up some parts in the registry with the keyword Metal. It seems like there are not many that we can use. How is everyone else going?
From Cheolwon Choi, 3rd of August
Here is a schematic diagram of biosensor. Your opinions are very welcome.
From Young Byun, 1st of August
Send me your photos and a short description of yourself by this weekend. Mottos and your message to iGEM are very welcome. Have a terrific weekend !!!
From Cheolwon Choi, 31st of July
Okay, guys. Here is a sum up of our votes, though I don't understand why you sent your votes through private mails. Our focus would be "Bacteria Array" and "Bacteria Compass."
| Title | Voters |
|---|---|
| Geosmin Absorber | Young Byun |
| Bateria Array | Hansung Roh, Young Byun, Cheolwoo Lee, Cheolwon Choi |
| Microdeisel | Cheolwon Choi |
| Keratin | Cheolwoo Lee |
| Biosticker | Kina Jeon |
| Mussel Glue | Kina Jeon |
| Morse Code | Yoonji Kim |
| Bacteria Compass | Yoonji Kim, Yeonjin Kong |
| Bacteira Pencil | Yeonjin Kong |
Mascot Design : Yeonjin Kong, Yoonji Kim, Kina Jeon
Parts Study : Young Byun, Hansung Roh
Circuit Design : Hyukjin Ko, Cheolwon Choi
Brocheure : Cheolwon CHoi
Those who couldn't attend our meeting, help out any groups you would like lend a hand on. As for the parts study, make a list with Excel or whatever you would like to use, and explain how they work. Biobrick numbers registered on iGEM is also crucial.
I am planning of a party? to encourage our team. Post me your convenient time.
P.S. I almost forgot. Use Google group unless it's something VERY private !!! Don't send your mails directly to me. :)
From Hansung Roh, 26th of July
I have my votes for "Bacteria Array."
I also read an interesting article on Bacillus pasteruii which concretes sand into cement using calcium carbonate. It was first invented to solidify bedrocks along beaches but is now planned to build sand walls around deserts to prevent its growth.
These are the links:
http://cedb.asce.org/cgi/WWWdisplay.cgi?0611182
http://www.popularmechanics.co.za/content/news/singlepage.asp?key=229
http://www.news.ucdavis.edu/search/news_detail.lasso?id=8040
http://www.environmentalgraffiti.com/featured/bacteria-sahara-desertification/11121
From Cheolwon Choi, 28th of July
I made a list of our ideas accumulated till now. You have two votes. Choose two subjects that you think are feasible, interesting, and suitable for synthetic biology. Have your votes sent by Wednesday.
| Category | Subject | Contents |
|---|---|---|
| Environment | Metal Absorber | By attaching metal detector on flagella, we can induce the bacteria to move toward metal and mingle |
| Geosmin Absorber | Detect and remove geosmin below 0.01ug | |
| Bacteria Array | Detect specific chemicals or substrates using bacterias as biosensors | |
| Oil Degradation | Removal of oil by lipase | |
| Food / Energy | Micro Diesel | Production of diesel with bacterias |
| Keratin | Removal of keratin, which is not easily degraded, through keratinase | |
| Hygiene | Surface active reagent | Production of lung surfactant with biobrick |
| Insect Repeller | Hinder insects incursion through insect pheromone | |
| Plaque | Removal of plaques on teeth by bacteria | |
| Bio Sticker | Histamin sensor, using human histamine receptor | |
| Etc. | Mussel Glue | Construction of a biobrick that can produce biosynthetic glue |
| Morse Code | Make Morse Code with bacteria signals using circuits that make cycles | |
| Compass | Build a compass with iron-philic bacteria | |
| Bio Pencil | A pencil consisted of bacteria lead which you can write freely but can be read within a limited time |
From Cheolwoo Lee, 26th of July
Remember Kina's idea about geosmin? This is an article about geosmin determined as a criteria for choosing drinkable water. http://www.iipc.co.kr/notice/notice1_view.asp?id=notice&bbsid=notice1&board_id=notice&ref=111&step=1&RefLevel=0¤t_page=
From Yeonjin Kong, 26th of July
As I was looking through some papers, I read about mussels. A Korean science team devloped bio-glue from mussels that live attatched to rocks in the ocean. The problem is that the amount produced is scarce, which we can solve by using microbiology. Bioglue seems attractive.
From Professor Ingeol Choi, 25th of July
To team members. I have read all the mails and they are great. Try to develop more ideas on the environment part.
- Make a list of ideas and categorize them.
- Do not think about if they would work or not, yet. That's not brainstorming.
- If you want to be more specific, think about what parts or circuits you could use for the idea.
Have you all studied what synthetic biology is? Check out http://partsregistry.org/Catalog. It might help you out. The file included contains my suggestions about your ideas. Have a nice day everyone.
From Hanseung Roh, 22nd of July
http://parts.mit.edu/igem07/jam07media/Jam07_Glasgow.pdf
Check it out.
From Cheolwon Choi, 22nd of July
Hey guys. I wish there were more exchange of ideas by Google. Don't keep your ideas to yourself. Share them with others and discuss freely. Don't think that your ideas are not worth it.
From Cheolwon Choi, 21st of July
There are people still confused about how to use Google groups. It is extremely SIMPLE. Send your mail to synbiogroup@googlegroups.com and all the team members recieve your mail. Yeap, that's all you need to do.
From Young Byun, 20th of July
Scrombroid food poisoning is the 2nd prevalent food posioning among fish and its main cause is histamine. Histidine which exists in all fish turns into histamine by histidine decarboxylase at temperature above 16 degrees celcius. Histmine is also noteworthy as a substrate for the expression of alergic reactions.
The basic mechanism is using "Band defect network" that was introduced in 'Towards Logical Designs in Biology.' GFP expression is repressed at low levels of histamine but strongly expressed at above threshold level. By this way, we can tell if the fish is edible or not. We are using a well known circuit here, so it might be a nice try.
I thought about the ideas our team produced. I think Bacteria Array is fascinating. But as for the aroma producing bacteria, can the small amount of fragrance produced overwhelm the stink of rotting food?
From Cheolwon Choi, 20th of July
If there is a way, I want to develop a way to increase the survival rates of diesel producing bacterias. This idea is also good for raising funds if we succeed! Can we also make bio gasolines?
From Kina Jeon, 20th of July
There is a molecule called Geosmin produced by microbes in water. It giveas the water a dirt flavor, an important factor for drinking water. We could build a bacteira that can sense geosmin and trap it, like the one Chiva university did on iGEM 2007.
From Hansung Roh, 19th of July
As I was searching the text books, I thought of making a "Bacteria Array," similar to DNA array. It can be built to sense specific substrates in a compound.
From Cheolwon Choi, 19th of July
Gees. It poured cats and dogs during the weekends. Your ideas are due Monday. I appreciate your full participation.
From Simin Kim, 15th of July
Hey people. How's an aroma producing bacteria? If we can make a bacteria that can produce fragrance by using wasted food, we might not need to buy Febreeze anymore! :)
From Cheolwon Choi, 10th of July
Hi everyone. The two subjects that we thought were good turned out to be not that suitable for iGEM. We need MORE ideas!!! We also need a clearer concept on synthetic biology. These are homeworks due to our next meeting, 17th of July.
1) https://2009.igem.org/Instructional_Videos Watch the videos.
2) Study the videos on iGEM 2009 home and tell us what you learned.
3) Divide our team into groups for each subject.
4) We need to learn more about Wiki. Wiki seems to take great part in judging. Although we are not that good in English, try to post your ideas. Try to visit our team Wiki everyday.
5) Please!!! Post me if you can't come.
From Cheolwon Choi, 5th of July
This mail has a linked file that contains the lists of our team member's e-mails and contacts.
From Cheolwon Choi, 4th of July
Hi everyone. We narrowed our ideas to two subjects, which are "Morse Code" and "Bio Sticker." If you would like to share any other ideas, you are more then welcome. Let's discuss about specific models for these two subjects at the upcoming meeting. Our next meeting is on 10th of July, 3 p.m. I recommend you think about at least one of the two subjects and how they should be developed. Tell me your schedules via mail so that I can fix our meeting time to the most suitable for all. I would like to share my experience at the iGEM workshop at Dongkyung about Wiki and other stuffs that you might be interested, so bring your notebooks. As I said before, prepare a specific model for the meeting. Try to document your ideas so they could be shared with others. A specific model should include these:
1. How will the chosen subject be used?
2. What are its limits?
3. What materials would be needed?
4. What former knowledge would be required to process the subject?
5. Why did you choose the subject?
6. What contributions can you make?
7. Etc.
See you next Friday.
Korean stuffs
- [http://openwetware.org/wiki/IGEM:KU_Seoul/2009/Notebook/iGEM2009 Lab Notebook@Openwetware (한글)]
