Formal Lab Session - 17th September 2009
Stochastic switch team
Today we ran the minipreps for sac and ara transformed cells however- perhaps due to poor miniprep procedure- the sac test digest yeilded poor results, however the ara transformants miniprep gave a ~200bp band along with the backbone in 8/12 lanes:
Midi prep cultures were set up using cultures corresponding to lanes 1 and 3 for ara.
To test whether the poor sac result was due to the miniprep more miniprep cultures were set up to do tomorrow.
Today also we did fragment preps for Goksels sspb the ara fragment, and a cwlJ fragment for Hanny:
Metal Sensing Team
Introduction
In yesterday's lab session the Metal Sensing team carried out mini-preps on the 12 colonies of potential cotC-GFP-smtA E. coli transformants and analysed them. Despite them showing incorrectly sized bands when digested and ran on gel, the team proceded to carry out preparations to midi-prep colony 8 on the basis of consistency of the band patterns and the theory that the digests may not have worked properly. The midi-prep will be done today.
The team also prepared to ligate the cadA promoter to the BBa_J33206 BioBrick (which contains the arsR gene and arsR binding site and has the promoter cut out) but only got as far as digesting the two fragments. The cadmium-sensor has been put on hold as of the General Meeting on 16/09/09.
Practical Outline
Procedure
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