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From 2009.igem.org
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- 16:12, 31 August 2009 (diff | hist) N File:Ligation voulume calcuator 090831 1.xls (top)
- 16:12, 31 August 2009 (diff | hist) N File:Assembly volume calcuator090831 1.xls (top)
- 16:11, 31 August 2009 (diff | hist) Uppsala-Sweden/31 August 2009 (→Transformation)
- 15:11, 31 August 2009 (diff | hist) N File:Digest purification measur 09831.txt (top)
- 15:11, 31 August 2009 (diff | hist) Uppsala-Sweden/31 August 2009
- 13:20, 31 August 2009 (diff | hist) N File:Digestion(excert)090831.xls (top)
- 13:20, 31 August 2009 (diff | hist) Uppsala-Sweden/31 August 2009
- 13:05, 31 August 2009 (diff | hist) N File:20090831 Adh2Y.jpg (top)
- 13:04, 31 August 2009 (diff | hist) N Uppsala-Sweden/31 August 2009 (New page: {{Uppsala-Sweden Template}} ==Adh2Y PCR== Gel picture, one nice band. Image:20090831_Adh2Y.jpg to be annotated --~~~~)
- 21:42, 30 August 2009 (diff | hist) Team:Uppsala-Sweden/Team
- 14:07, 30 August 2009 (diff | hist) N File:Assembly volume calcuator 090830 2.xls (top)
- 14:07, 30 August 2009 (diff | hist) N File:Assembly volume calcuator 090830 1.xls (top)
- 14:06, 30 August 2009 (diff | hist) N Uppsala-Sweden/30 August 2009 (New page: {{Uppsala-Sweden Template}} ==Assembly== Today we well assemble the constructs RBS-pdc_RBS-adh2Z, RBS-pirB-Terminator, PpetE-RBS. Volumes in these files [[Media:Assembly volume calcuator...)
- 16:11, 29 August 2009 (diff | hist) N File:Digestion 090829.txt (top)
- 16:11, 29 August 2009 (diff | hist) Uppsala-Sweden/29 August 2009 (top)
- 16:10, 29 August 2009 (diff | hist) Uppsala-Sweden/29 August 2009
- 13:06, 29 August 2009 (diff | hist) N File:Plasmidprep 090829 2.txt (top)
- 13:05, 29 August 2009 (diff | hist) N File:Plasmidprep 090829.txt (top)
- 13:05, 29 August 2009 (diff | hist) N Uppsala-Sweden/29 August 2009 (New page: {{Uppsala-Sweden Template}} ==Plasmid prep of RBS + PDC construct== Measurement of the plasmid prep from the RBS + PDC construct, 50 times dilution. Media:Plasmidprep_090829.txt ==Te...)
- 10:41, 28 August 2009 (diff | hist) Uppsala-Sweden/27 August 2009 (top)
- 17:29, 27 August 2009 (diff | hist) Uppsala-Sweden/27 August 2009
- 17:28, 27 August 2009 (diff | hist) N Uppsala-Sweden/27 August 2009 (New page: {{Uppsala-Sweden Template}} ==Assembly evaluation== Here are he images of the evaluation gel f yesterdays assembly: Upper Image:20090827 colonypcr_1 Lower [[Image:20090827 colony...)
- 17:28, 27 August 2009 (diff | hist) N File:20090827 colonypcr 2.jpg (top)
- 17:27, 27 August 2009 (diff | hist) N File:20090827 colonypcr 1.jpg (top)
- 20:33, 26 August 2009 (diff | hist) Uppsala-Sweden/26 August 2009 (top)
- 10:33, 25 August 2009 (diff | hist) N Uppsala-Sweden/24 August 2009 (New page: {{Uppsala-Sweden Template}} ==Preparaing competent cells== Today 160 2 ml tubes with 50 µl top ten cells each were prepared and put in the - 80 freezer according to the [http://openwetwa...) (top)
- 19:34, 17 August 2009 (diff | hist) N Uppsala-Sweden/17 August 2009 (New page: {{Uppsala-Sweden Template}} ==Top10 competent cells, making== As our earlier competent cells weren't working very well we decided to make new ones and in the process switch to the seeming...)
- 15:45, 13 August 2009 (diff | hist) m Uppsala-Sweden/13 August 2009
- 15:44, 13 August 2009 (diff | hist) N Uppsala-Sweden/13 August 2009 (New page: {{Template Uppsala-Sweden}} ==Transformation of ADH2 from z.mobilis, continued== Unfortunatly we only got two (!!) colonies from Adh2 z. These are most likely to be contamination as one w...)
- 15:28, 13 August 2009 (diff | hist) m Uppsala-Sweden/12 August 2009
- 15:35, 11 August 2009 (diff | hist) N Uppsala-Sweden/11 August 2009 (New page: {{Uppsala-Sweden Template}} ==Evaluation of competent cells== Due to the run of bad transformations we've experienced we decided to evaluate the quality of our cells. We used our previosl...)
- 15:20, 11 August 2009 (diff | hist) m Uppsala-Sweden/10 August 2009
- 20:00, 1 August 2009 (diff | hist) Uppsala-Sweden/31 July 2009 (top)
- 19:47, 1 August 2009 (diff | hist) Uppsala-Sweden/30 July 2009 (top)
- 19:45, 1 August 2009 (diff | hist) Uppsala-Sweden/31 July 2009
- 19:38, 1 August 2009 (diff | hist) Uppsala-Sweden/30 July 2009
- 13:57, 30 July 2009 (diff | hist) N Uppsala-Sweden/29 July 2009 (New page: {{Uppsala-Sweden Template}} '''Competent cell preperation''' We made CCMB80 buffer and inoculated four 2 ml cultures of competent E.coli as given by: [http://openwetware.org/wiki/TOP10_...) (top)
- 13:49, 30 July 2009 (diff | hist) N Uppsala-Sweden/30 July 2009 (New page: {{Uppsala-Sweden Template}} '''kivd adhII pdc PCR''' Today we begun the trials to extract the kivd gene from Lactococcus lactis, and adhII and pdc from S.cerivase. We will try to extrac...)
- 11:53, 23 July 2009 (diff | hist) N Uppsala-Sweden/23 July 2009 (New page: {{Uppsala-Sweden Template}} '''Butanol survival''' OD Mesurment 11:48 Marked growt in 0% Degeneration of OD in higher concentrations --~~~~) (top)
- 11:51, 23 July 2009 (diff | hist) Uppsala-Sweden/22 July 2009 (top)
- 11:50, 23 July 2009 (diff | hist) N Uppsala-Sweden/22 July 2009 (New page: {{Uppsala-Sweden Template}} '''Butanol Survival''' OD Measurment 13:31 Growth of 0% others at stand still --~~~~)
- 11:47, 23 July 2009 (diff | hist) Uppsala-Sweden/21 July 2009
- 11:43, 23 July 2009 (diff | hist) N File:Butanol+survival+test+conc090721.xls (Experiment set up butanol suvival 090721) (top)
- 11:40, 23 July 2009 (diff | hist) Uppsala-Sweden/21 July 2009
- 11:29, 23 July 2009 (diff | hist) Uppsala-Sweden/20 July 2009 (top)
- 12:03, 19 July 2009 (diff | hist) Uppsala-Sweden/15 July 2009 (top)
- 12:02, 19 July 2009 (diff | hist) N Uppsala-Sweden/15 July 2009 (New page: '''Determination of linear range OD culture measurement.''' Start culture was diluted 1 2 4 8 16 32 64 128 parts to final volume 200 µl on 96-well plate with BG-11 growth media, triplica...)
- 11:55, 19 July 2009 (diff | hist) Uppsala-Sweden/17 July 2009
- 11:21, 19 July 2009 (diff | hist) Uppsala-Sweden/16 July 2009
- 11:20, 19 July 2009 (diff | hist) N Uppsala-Sweden/16 July 2009 (Start of ethanol survival experiment.)
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