"
User contributions
From 2009.igem.org
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
- 17:11, 30 July 2009 (diff | hist) N File:Vgem-colonyPCR-E0240.JPG (top)
- 15:16, 30 July 2009 (diff | hist) Team:Virginia/30 July 2009 (→Overnight Results)
- 15:14, 30 July 2009 (diff | hist) N Team:Virginia/30 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== *Gel of **Colony PCR of I20269 and I20270 ***Photo to be added **Colony PCR of E0240 ***Photo to be added **Cut vectors for ...)
- 15:11, 30 July 2009 (diff | hist) Team:Virginia/29 July 2009 (→Today) (top)
- 18:44, 27 July 2009 (diff | hist) Team:Virginia/23 July 2009 (→Today) (top)
- 18:36, 27 July 2009 (diff | hist) Team:Virginia/23 July 2009 (→Today)
- 18:34, 27 July 2009 (diff | hist) N File:PCS-HA.JPG (top)
- 18:34, 27 July 2009 (diff | hist) Team:Virginia/23 July 2009 (→Today)
- 18:30, 27 July 2009 (diff | hist) N File:PCS-HA.tif (Product of PCS-HA PCR) (top)
- 18:22, 27 July 2009 (diff | hist) Team:Virginia/20 July 2009 (→Today) (top)
- 14:31, 27 July 2009 (diff | hist) N Team:Virginia/27 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== All platings blank again ==Today==)
- 19:51, 23 July 2009 (diff | hist) Team:Virginia/23 July 2009 (→Today)
- 19:31, 23 July 2009 (diff | hist) N Team:Virginia/23 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== *Ligated A09-10 **ABP-HA E,P **pSB1C3 E,P **Versions 1, 2, and 3 reflect different ratios of vector to insert. 1:2, 1:6, ...)
- 19:17, 23 July 2009 (diff | hist) N Team:Virginia/22 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== Digested pSB1C3 E,P) (top)
- 19:13, 23 July 2009 (diff | hist) Team:Virginia/21 July 2009 (→Today) (top)
- 14:17, 21 July 2009 (diff | hist) N Team:Virginia/21 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== Results from transformation *Had colonies **PCS(2) **pSB1A3 **pSB1C3 **pSB1k3 **pSB3K3 *No colonies **-Control **+Control **pSB1T3 ==T...)
- 21:04, 20 July 2009 (diff | hist) Team:Virginia/20 July 2009 (→Today)
- 16:33, 20 July 2009 (diff | hist) N Team:Virginia/20 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== *Transformations failed ==Today== *Ran gel of Colony PCR **Photo coming *Ran gel of A09-7 and A09-8 DNA **Both lanes blank *Cut JW13002...)
- 16:30, 20 July 2009 (diff | hist) N Team:Virginia/18 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== *Colony PCR of PCS in JW3469 and JW3470 *Repeated transformation of A09-7 and A09-8) (top)
- 17:11, 17 July 2009 (diff | hist) N Team:Virginia/17 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== Transformations were blank ==Today== *Ran colony PCR of PCS in JW3469 and JW3470 *Transformed DH5alphas with A09-7 and A09-8) (top)
- 13:57, 16 July 2009 (diff | hist) N File:Colonypcrpcs.tif (top)
- 13:53, 16 July 2009 (diff | hist) N Team:Virginia/16 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== Strange results from plating *Had colonies **JW3469- (+) control **JW3469-PCS **JW3470- (-) contol **JW3470- PCS *No Colonies **JW3469...) (top)
- 20:51, 15 July 2009 (diff | hist) N Team:Virginia/15 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== *Ligated A09-7 and A09-8 *Transformed **A09-7 in DH5alpha **A09-8 in DH5alpha **PCS miniprep in JW3469 **PCS miniprep in JW...) (top)
- 14:52, 15 July 2009 (diff | hist) N Team:Virginia/14 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== Both negative and positive controls blank for PCS in DH5alpha but colonies on PCS plate. ==Today== *Glycerol stocks of PCS in DH5alph...) (top)
- 14:45, 15 July 2009 (diff | hist) N Team:Virginia/13 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== *Retransformed PCS into DH5alpha *PCRed HA tag onto ABP *Gel purified ABP-HA) (top)
- 14:42, 15 July 2009 (diff | hist) N Team:Virginia/10 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== *New plates (A) *New chemically competent cells) (top)
- 14:41, 15 July 2009 (diff | hist) Team:Virginia/9 July 2009 (→Overnight Results) (top)
- 14:37, 15 July 2009 (diff | hist) N Team:Virginia/9 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== Ran SDS-Page and Coomassie stained.)
- 18:21, 1 July 2009 (diff | hist) N Team:Virginia/1 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== Ran 2 mM arsenic growth curve *k12 *JW3469 *JW3470) (top)
- 16:39, 30 June 2009 (diff | hist) Team:Virginia/30 June 2009 (→Today)
- 14:48, 30 June 2009 (diff | hist) Team:Virginia/30 June 2009 (→Today)
- 14:47, 30 June 2009 (diff | hist) N Team:Virginia/30 June 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== Ran growth curve at 2mM arsenic concentration.)
- 13:53, 26 June 2009 (diff | hist) N Team:Virginia/26 June 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== *No colonies **A09-3 **A09-4 ==Today==)
- 13:52, 26 June 2009 (diff | hist) N Team:Virginia/25 June 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== *Cut, ligated, and transformed **ABP-A09-3 **ArsR/RBS OFP-A09-4 *DNA was gel purified after cutting) (top)
- 14:13, 24 June 2009 (diff | hist) Team:Virginia/24 June 2009 (→Overnight Results) (top)
- 13:56, 24 June 2009 (diff | hist) N Team:Virginia/24 June 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== *Results of yesterdays platings **ArsR had colonies **Cell death had colonies **Med I no colonies ==Today==)
- 15:46, 22 June 2009 (diff | hist) N Team:Virginia/22 June 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== *Cut expression vectors **J13002 (S,P) **J04500 (S,P) *Transformed DH5-alphas with **F VII **Cell death gene k124017 **Ars...)
- 14:33, 18 June 2009 (diff | hist) N Team:Virginia/18 June 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== *Ran growth curves in 2mM arsenate and arsenite. **K12 **JW3469 **JW3470) (top)
- 14:20, 17 June 2009 (diff | hist) N Team:Virginia/17 June 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== *Expression vectors had colonies **J04500 **J13002 *Reporters had colonies **OFP **GFP **RFP had about half colonies which grew on the ...)
- 14:11, 17 June 2009 (diff | hist) Team:Virginia/16 June 2009 (→Today) (top)
- 15:37, 12 June 2009 (diff | hist) N Team:Virginia/11 June 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== *Set up growth curves in arsenic *Made knockouts chemically competent **JW3469 **JW3470 *Did serial dilutions of optical den...) (top)
- 15:36, 12 June 2009 (diff | hist) N Team:Virginia/12 June 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== Serial dilutions were all lawns ==Today== *Ran growth curves in arsenic **JW3469 **JW3470 **K12)
- 17:58, 10 June 2009 (diff | hist) N Team:Virginia/10 June 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== *No colonies from plasmid transformations ==Today== *Re-transformed plasmids *Prepared -20 glycerol stocks of knockouts JW3469 and JW3...)
- 17:55, 9 June 2009 (diff | hist) N Team:Virginia/9 June 2009 (New page: {{Template:VirginiaNotebook}} ==Today== *More growth curves. **K12 **DH5a **JW3469 **JW3470 *Confirmed A09-1 unsuccessful *Redoing A09-001. **A09-1(2) *Made fresh Tetracyclin *5ul broths ...)
- 15:36, 3 June 2009 (diff | hist) Team:Virginia/3 June 2009 (→Today)
- 14:13, 3 June 2009 (diff | hist) Team:Virginia/3 June 2009 (→Today)
- 14:13, 3 June 2009 (diff | hist) Team:Virginia/3 June 2009 (→Today)
- 14:11, 3 June 2009 (diff | hist) N Team:Virginia/3 June 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== Results of plating *psB1T3 Nothing *psB1C3 Small colonies *psB4A5 lawn *psB1A3 lawn *psB1K3 Small colonies ==Today==)
- 20:13, 1 June 2009 (diff | hist) Team:Virginia/1 June 2009 (→Today)
- 18:02, 1 June 2009 (diff | hist) N Team:Virginia/1 June 2009 (New page: Used the total genome extract from friday to do PCR run with ArsC primers. *used low concentration of MgCl .7 mM.)
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
