Team:EPF-Lausanne/Protocols/Illumination

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Illumination Process



Material

  • LED blue 3mm (Distrelec)
  • LED750-5mm (Roithner LaserTechnik)
  • AC Power supply



Procedure

The initial cell culture is made in dark state. We then illuminate them in the incubator, with constant shaking. To do so, we use small erlenmeiers to have a sufficient amount of culture for the experiments and so that the cells are in a sufficient medium to grow. We aslo use the red LEDs to inactivate the LovTAP while loading our samples for further experiments.

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