Conditioned Reflex Mimicking in E.coli
We are engineering our E.coli cells to process the correlation information of two environmental signal, similar to the process of classical conditioning in higher organisms. In our circuits we use a bistable switch as the memory module to represent the memory state; we also constructed a series of AND gates which can sense conditioned and unconditioned signals, and output a repressor protein to change the memory state of the bistable switch. In this way, our E.coli cells can convert the information on the concurrence of two signals to its memory. After the memory module is switched, given the conditioned stimulus, E.coli will activate its reporter module and thus exhibit the "conditioned response".
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