Notebook > Assembly > Assembling : bistable-> pSB1A2 & pSB6A1
2009.10.1
10:00
Double-enzyme digestion:
| Plasmid(bistable on pSB4K3) | 5μl
|
| SpeI | 1μl
|
| PstI | 1μl
|
| Buffer H | 2μl
|
| ddH2O | 11μl
|
14:00
Gel purification
16:00
Ligation:
Vector: Bistable
Insert: T7-CI(1H, 2M, 2I, 2G, 2K, 1J)
Ligase: 1μl
Buffer: 1μl
18:00
Transformation
2009.10.2
10:00
Bacteria culture:
Bistable-T7p-CI(1H, 2M, 2I, 2G, 5N, 1J)
24:00
Mini-prep:
Bistable-T7p-CI(1H, 2M, 2I, 2G, 2K, 1J)
2009.10.3
09:00
Double-enzyme digestion: Bistable-T7p-CI(1H, 2M, 2I, 2G, 2K, 1J)
16:00
Ligation:
Vector: Bistable on 1O and on 2K
Insert: T7p-CI(1H, 2M, 2I, 2G, 2K, 1J)
20:00
Transformation
2009.10.4
09:00
Bacteria culture:
1O-Bistable-T7p-CI-2K, 1O-Bistable-T7p-CI-2G, 1O-Bistable-T7p-CI-2M, 12K-Bistable-T7p-CI-2I, 12K-Bistable-T7p-CI-1H, 12K-Bistable-T7p-CI-1J
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