Team:SJTU-BioX-Shanghai/Parts
From 2009.igem.org
Revision as of 02:42, 21 October 2009 by Pnstontale (Talk | contribs)
Project introduction. Inspired by the natural regulator of circadian bioclock exhibited in most eukaryotic organisms, our team has designed an E.coli-based genetic network with the toxin-antitoxin system so that the bacterium oscillates between two states of dormancy and activity (more...)
Parts & devices
- Go to [http
- //partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=SJTU-BioX-Shanghai PartsRegistry] to see our submited parts.
Almost all of our parts have been detected after enzyme digestion. The following DNA Electrophoresis pictures prove our parts to be accurate and reliable.
- Lane2: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185000 BBa_K185000] RelE toxin+Rbs30 311bp OK
- Lane3: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185001 BBa_K185001] Arabinose-induced RelE Generator 1791bp Failed
- Lane4: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185002 BBa_K185002] Lon Protease+Double terminater 2492bp OK
- Lane5: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185003 BBa_K185003] RelE toxin+Lon proteas 2827bp OK
- Lane6: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185004 BBa_K185004] RelE toxin+Double terminator 443bp OK
- Lane3: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185028 BBa_K185028] RelB+Double-terminator 395bp OK
- Lane3: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185007 BBa_K185007] Promoter116+RBS34+lLacI+Double-terminator+Plac+RBS31 1514bp OK
- Lane5: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185016 BBa_K185016] Promoter118+RBS34+LacI+Double-terminator+Plac+RBS31 1514bp OK
- Lane15: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185018 BBa_K185018] Promoter118+QPI+RBS30+RelB+Double-terminator 1454bp OK
- Lane17: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185036 BBa_K185036] Promoter110+QPI+RBS34+RelB+Double-terminator 1433bp OK
- Lane20: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185011 BBa_K185011] Promoter116+QPI+RBS31+RelB+Double-terminator 1435bp OK
- Lane13: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185053 BBa_K185053] Promoter116+RBS34+LacI+Double-terminator+pPlac+RBS31+RelE+RBS30+Lon protease+Double terminator 4347bp OK
- Lane10: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185012 BBa_K185012] Promoter116+RBS34+LacI+Double-terminator+Plac+RBS34 1347bp OK
- Lane12: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185019 BBa_K185019] Promoter118+RBS34+LacI+Double-terminator+Plac+RBS34 1512bp OK
- Lane 2&3: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185045 BBa_K185045] Composite J23110+Q01121 1416bp OK
- Lane 4&5: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185046 BBa_K185046] Composite J23110+Q01121 1416bp OK
- Lane 6&7: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185042 BBa_K185042] Composite J23110+Q04510 1030bp OK
- Lane 8&9: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185043 BBa_K185043] Composite J23116+Q04510 1030bp OK
- Lane 10&11 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185044 BBa_K185044] Composite J23118+Q04510 1030bp OK
- Lane 5&6&7: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185037 BBa_K185037] Composite J23116+Q04510+B0031 1052bp OK
- Lane 8-12: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185038 BBa_K185038] Composite J23116+Q04510+B0034 1050bp OK
- Lane 15&17&18&19: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185039 BBa_K185039] J23118+Q04510+B0030 1053bp OK
- Lane 20&21: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185040 BBa_K185040] J23118+Q04510+B0031 1052bp OK
- Lane 2-8: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185034 BBa_K185034] Device J23116+Q04510+B0034+K185048 1433bp OK
- Lane 4: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185005 BBa_K185005] Generator J23116+Q04510+B0034+K185048+K185005 3298bp OK
- Lane 2-9: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185051 BBa_K185051] Composite J23110+B0034+BBa_K185008+BBa_K185009 2748bp PCR analysis (plasmid backbone added) OK
- Lane 2, 4, 6, 7: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185052 BBa_K185052] Regulatory 1342bp PCR analysis (backbone sequence added) OK
- Lane 2,3: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185009 BBa_K185009] Device 2047bp OK
- Lane 1,3,5,6: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185031 BBa_K185031] intermediate 1104bp OK
- Lane 4,5,6,7,8,9,10,11: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185033 BBa_K185033] inverter 1514bp OK