Transformation of week-long Ligation of promoter library and vector pCS26
Promoter Library product (from primer extension PCR) was ligated with pCS26 vector for 9 days with T4 Invitrogen Ligase at room temperature (~21oC). This product was transformed into both XL Gold Ultracompetent and Top 10 cells.
Grew these cells on LB plates with Kan resistance at 37oC for 16 hours.