Team:Newcastle/Labwork/10 August 2009

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Lab Session 10/08/09

Stochastic Switch Team

Today we pepared gfp-rrnb plasmids using midiprep. We followed GenElute's midiprep protocol.

We had two E.coli cultures inoculated with gfp-rrnb left overnight. Although they had teh sampels, we treated them individually.

We centrifuged the tubes at 16000rpm for 30 minutes before adding ethanol to rinse the DNAs. We then centriguged the tubes again for 10 minutes at 16000rpm.

We evaporated the tubes fro 5 minutes at the end.

Results

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Team Newcastle iGEM 2009 08 10 Sample 2.png

Chassis team

  • Created germination solution without lysozyme.
  • Borrowed Em from Colin Harwood's lab.
  • Created a new MgCl Stock solution, as old one had bits of matter floating in it.
  • Created KPh stock solution.
  • Autoclaved solutions.
  • Made 250ml LB and 250ml H20, autoclaved.
  • Inoculated LB with Em and Kan, then poured plates.



News

Events

Social Net

  • Newcastle iGEM Twitter
  • [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
  • [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
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