Team:Newcastle/Labwork/13 August 2009

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Revision as of 00:21, 16 August 2009 by Janehsy (Talk | contribs)


Lab Session 13/08/09

Sporulation Tuning/Chassis Team

Yesterday's germination attempt seems to have worked, but results are not yet clear. This may have been due to a calculation error for the lysozyme, where instead of adding 40ul of stock lysozyme per ml of buffer solution, 4ul of stock lysozyme was added instead.

Today we are trying to transform Bacillus subtilis with gfp-rrnb integration vector. Metal sensor team kindly inoculated B. subtilis cells into flask tubes and placed them into the shaking incubator. The overnight cultures were labelled 1, 2 and 3, while the control was labelled as 4.





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