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Team:Warsaw/Sources
From 2009.igem.org
Sources of natural bricks
Below we present sources of bricks prepared by our team.
Invasiveness bricks
Most of bricks connected with bacteria invasiveness were acquired from following organisms:
| organism | genomic sequence details | acquired genes |
|---|---|---|
| Salmonella enterolitica ser. typhimurium LT21 | [http://www.ncbi.nlm.nih.gov/nuccore/NC_003197 NC_OO3197] | PhoP, PhoQ, MgtC promoter |
| Listeria monocytogenes EGD-E2 | [http://www.ncbi.nlm.nih.gov/nuccore/NC_003210 NC_003210] | listeriolysin, internalinA |
| Yersina enterolitica O:8 (8081)3 | [http://www.ncbi.nlm.nih.gov/nuccore/AM286415 AM286415] | invasin |
Other bricks
| name of the brick | sequence details | source |
|---|---|---|
| CDS of p53 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177016 BBa_K177016] | human4 |
| CDS of bax | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177027 BBa_K177027] | human 5 |
| mitochondrial targeting signal from the Cox gene | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177028 BBa_K177028] | human6 |
| secretion system | Escherichia coli7 |
1 Genomic DNA from this strain was a generous gift from ...
2 Genomic DNA from this strain was a generous gift from prof. Jacek Bielecki, PhD, [http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=528&b_2501=5 Laboratory of Applied Microbiology], Faculty of Biology, University of Warsaw
3 Genomic DNA from this strain was a generous gift from ...
4 Plasmid carrying p53 gene was a generous gift from Dawid Walerych, PhD, from the [http://www.iimcb.gov.pl/zylicz_lab.php Lab of Molecular Biology], International Institute of Molecular and Cell Biology in Warsaw
5 Plasmid carrying bax gene was a generous gift from Łukasz Borowski, MSc, from the [http://www.igib.uw.edu.pl/index.php?id=80 Institute of Genetics and Biotechnology], University of Warsaw.
6 Mitochondrial targeting signal was amplified from the [http://www.clontech.com/images/pt/PT3730-5.pdf pAcGFP-mito] vector
7 Plasmids carrying essential elements of mentioned secretion system were a generous gift from Carlos A. Guzman; [http://www.helmholtz-hzi.de/en/research/research_groups/vaccinology_and_applied_microbiology/vaccinology/ Department of Vaccinology and Applied Microbiology], Helmholtz Center for Infection Research
