Team:Newcastle/Meetings/16 April 2009

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Contents 1 Instructor Meeting 16 April 2009 1.1 Agenda of the Meeting 1.2 Action Points of the Meeting 1.3 Minutes of the Meeting

Instructor Meeting 16 April 2009

Students: Jess, James, Hanny, Goksel Instructors:

Apologies: Craig, Matt, Jane, Arun

Agenda of the Meeting

• More about flowchart/table/modelling
• iGEM wiki changes
  • Europe Workshop: Most likely May 30/31 in London, UK at Imperial College.
  • Lethbridge - Bacteria over Batteries - bacterial battery that uses photosynthesis to generate electricity.
  • EPF-Lausanne - Brainstorm of 10 ideas.

Action Points of the Meeting

• Need to show normal growth on diagram
• Model population dynamics
• Ask Nigel Robinson about Metallisation
• Make powerpoint more detailed (biobrick slides)
  • Name promoters that we are going to use
  • Put them in the right order
• Finding a homologue comK

Minutes of the Meeting

• Knock out comK, and replace with one from another organism
• Sigma D Motility, provides bi-stability
• Work in a mutant background for biofilm formation, if we use sinI/sinR stochastic switch. Knocking out biofilm 'genes'
• Use KinA
• Methylase, no GFP. Instead of DNA rearrangement?
• RNAs Restriction enzyme, instead of DNAase to prevent germination, but don't know how it will work
• recA mutants, can repair themselves
• Metallothiene incorporate with whole set of coat proteins of the spore
• GFP and RFP with smtA to see where it binds on the coat of the spore
• Knock out phosphotases rap. spo0E sporelation
• Natural switches instead of synthetic ones
• Streptococci Stochastisity Competence
• Sigma F (spore specific) to control DNA rearrangement
• Negative feedback to control smtA expression
• Control mntH using mntR, also controls mntABCD, lets in Fe
• Strong promoters - Sigma K, cotC, cotA - can be used for smtA expression
• Sigma D is expressed all the time, provides bi-stability
• May be able to use E-Coli biobricks for DNA rearrangement