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Team:Newcastle/Labwork/22 September 2009
From 2009.igem.org
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Formal Lab Session - 22nd September 2009
Stochastic switch team
Today we transformed E.coli JM109 cels with the overnight ligation product from yesterday using the promega protocol. We used the following controls:
- Tube 1: Backbone + insert + ligase (proper ligation)
- Tube 2: Backbone + ligase (control for background ligation level)
- Tube 3: Backbone + insert (control for ligase effectiveness)
These were left in the incubator for an hour and then plated out (2 plates per tube 200ul and 50ul) onto Amp + Tet LB plated and put in the 37C incubator overnight.
Today again we redid the digests of the sac miniprep however again there was no DNA present.
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
