From 2009.igem.org
|
|
December 2008
| M | T | W | T | F | S | S |
|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 |
|
| 8 | 9 | 10 | 11 | 12 | 13 | 14 |
|
| 15 | 16 | 17 | 18 | 19 | 20 | 21 |
|
| 22 | 23 | 24 | 25 | 26 | 27 | 28 |
|
| 29 | 30 | 31 | | | | |
|
|
March 2009
| M | T | W | T | F | S | S |
|
| | | | | | | 1 |
|
| 2 | 3 | 4 | 5 | 6 | 7 | 8 |
|
| 9 | 10 | 11 | 12 | 13 | 14 | 15 |
|
| 16 | 17 | 18 | 19 | 20 | 21 | 22 |
|
| 23 | 24 | 25 | 26 | 27 | 28 | 29 |
|
| 30 | 31 | | | | | |
|
|
April 2009
| M | T | W | T | F | S | S |
|
| | | 1 | 2 | 3 | 4 | 5 |
|
| 6 | 7 | 8 | 9 | 10 | 11 | 12 |
|
| 13 | 14 | 15 | 16 | 17 | 18 | 19 |
|
| 20 | 21 | 22 | 23 | 24 | 25 | 26 |
|
| 27 | 28 | 29 | 30 | | | |
|
|
May 2009
| M | T | W | T | F | S | S |
|
| | | | | 1 | 2 | 3 |
|
| 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|
| 11 | 12 | 13 | 14 | 15 | 16 | 17 |
|
| 18 | 19 | 20 | 21 | 22 | 23 | 24 |
|
| 25 | 26 | 27 | 28 | 29 | 30 | 31 |
|
|
|
|
|
|
|
|
|
November 2009
| M | T | W | T | F | S | S |
|
| | | | | | | 1 |
|
| 2 | 3 | 4 | 5 | 6 | 7 | 8 |
|
| 9 | 10 | 11 | 12 | 13 | 14 | 15 |
|
| 16 | 17 | 18 | 19 | 20 | 21 | 22 |
|
| 23 | 24 | 25 | 26 | 27 | 28 | 29 |
|
| 30 | | | | | |
|
|
|
|
Week from December 15th, to December 21st, 2008
December, 16th
Trasformation of Ligations
A5: BBa_Q0040 with BBa_I732017 (RBS-TETR-PTET-RBS-LACZ-TT)
A6: BBa_J23100 with BBa_Q0040 (PCON-RBS-TETR-TT-PTET )
- 1 ul A5 in Top10 Competent
- 1 ul A6 in Top10 Competent
Mix 80 ul of SOC with 20 ul X-GAL (40 mg/ml) and infect LB+Amp Agar plates.
- Add 250 ul SOC in 50 ul A5+ Top10
- Add 250 ul SOC in 50 ul A6+ Top10
1 hr incubation at 37°C (falcons with shaking 220 rpm)
Infect:
- X-GAL plate with 200 ul A5
- X-GAL plate with 100 ul A5
- Agar plate with 200 ul A6
- X-GAL plate with 20 ul of BBa_E0240 from glycerol stock (negative control).
Expected results:
- Plates 1 and 2 should develop blue colonies
- Plate 4 should remain colorless
16 hr incubation at 37°C.
"
