Team:Newcastle/Labwork/13 October 2009

From 2009.igem.org

Revision as of 18:09, 17 October 2009 by Goksel (Talk | contribs)

Today we decided not to characterize cotC+smtA biobricks since we won't have time to finish and we want to finish the lab work this week.

Preparation of parts to send to the registry

Measured the concentrations of the midipreps and prepared them to send to the parst registry sspB:64 ng/ul 

To parts registry: 6.4 ng/ul in 20ul

ara:34 ng/ul 

To parts registry: 3.4 ng/ul in 20ul

ara+sspB:87.5 ng/ul 

To parts registry: 8.75 ng/ul in 20ul

cotC+smtA:355.9 ng/ul 

To parts registry: 9 ng/ul in 20ul

cwlJ:76.9 ng/ul 

To parts registry: 7.69 ng/ul in 20ul

sleB:98.3 ng/ul 

To parts registry: 5 ng/ul in 20ul

swlJ + sleB:55 ng/ul 

To parts registry: 5.5 ng/ul in 20ul

Preparation of parts for sequencing

Prepared the primers for psB1AT3 (x2, prepared two sets)

Forward primer preparation: Total 30ul, 10pmol/ul 

Forward primer : 10ul (30pmol/ul)
PCR water: 20ul

Reverse primer preparation: Total 30ul, 10pmol/ul 

Forward primer : 7.5ul (40pmol/ul)
PCR water: 22.5ul
Placed into the box for sequencing parts
Retrieved from "http://2009.igem.org/Team:Newcastle/Labwork/13_October_2009"