2009.8.16
Test if the tetR promoter system works well in the low-copy backbone.
Get tetR+promoter+GRP from Wu Shuke.
16:40
Digest the promoter and reporter system.
| Total | 50μL
|
| Plasmids | 5μL
|
| EcoR1 | 1μL
|
| Pst1 | 1μL
|
| Buffer | 2μL
|
| ddH2O | 11μL
|
20:00
Electrophoresis to recycle the inserts.
The order of the samples: marker, digestion products, plasmids control.
Results:
Only the first one is correctly digested, recycle them.
2009.8.17
10:00
Link the inserts with vectors with has Kanamycin resistance.
17:50
Transformation.
19:30
Start to incubate.
2009.8.18
10:00
There are many colonies on the plate.
PCR colonies to test if they are correct.
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