Week from July 6th, to July 12nd, 2009

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July, 6th

• Digestion for:

• Gel run/cut and band purification.

• The purified DNA gave a good result at Nanodrop, but we decided to perform the entire work on BOL1 again because we were not sure that we had extracted a pure band.

• We stored R0011(E-X) DNA at -20°C.

• We infected 5 ml of LB + Amp with 8 ul of BOL1 glycerol stock to grow an overnight culture (37°C, 220 rpm).

Preparation of experiment with Tecan F200

• We picked a colony of B0030 plate (stored at +4°C) and infected 5 ml of LB + Amp.

• We also infected 5 ml of LB + Amp with 10 ul of A1 and J23100 glycerol stocks.

• We incubated the inocula overnight at 37°C, 220 rpm.

July, 7th

• Miniprep for BOL1 (X2).

• Digestion for BOL1(E-S)(X2).

• Gel run/cut/purification.

• Ligation:
  • A11 = BOL1(E-S) + R0011(E-X) in pSB1A2

• We incubated the ligation overnight at 16°C.

Preparation of experiment with Tecan F200

• We diluted 1:100 the overnight cultures of A1, B0030 and J23100.

• We incubated the diluted cultures for 5 hours (37°C, 220 rpm).

Experiment with Tecan F200

July, 8th

July, 9th

July, 10th

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