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Judging Comments

This year, despite our relatively ambitious project, our team achieved goals in several different areas.

In addition to successfully modelling, designing, characterising and entering our sporulation tuning kinA BioBrick (BBa_K174011) in the parts registry, we improved an existing BioBrick part (BBa_K174004) rendering it tightly regulated.

Futhermore, we helped the UQ-Australia iGEM2009 team by modelling their system.

We also modelled all the components of our project's system, and we designed and entered 16 other BioBrick parts and devices, ranking from a degradation controller system to a tightly regulated metal sensing promoter. We believe that several of the BioBrick parts we designed, in addition to our three favourite parts, are novel and can be used not only in the context of our project but in a variety of different systems.

Our degradation controller device is arabinose inducible and should be useful beyond our own project, to tightly regulate the levels of protein in a range of synthetic systems. Our device allows us to exert a third form of control over the expression level of a protein of interest, in addition to the transcriptional and translational regulation already widely used in Synthetic Biology.

Our sensitive cadmium promoter BioBrick is also novel since there is no previously available cadmium specific promoter. Our combinatorial approach, using two different transcriptional repressors is a tightly regulated promoter which can be used by any cadmium-sensitive system.