Team:TUDelft/Module 3 Why?
From 2009.igem.org
Why?
As mentioned in the project description, one important feature performed for our system is the reset of the signal once the message is received. The time between the signal (or self destructive plasmid/conjugation plasmid) has been received and the reset (or destruction of the plasmid) is an important parameter which will allow the signal to be send to the next cell (receiver) through the consecutive conjugation system before “losing” the message. Therefore, it is necessary to construct a device which will give enough time for the two subsequent events happen. This device is termed time-delay genetic circuit in this project. In a final version, the time-delay genetic circuit will have as input the entry conjugation plasmid into one cell and will deliver as output the expression of the endonuclease in charge of the destruction of the mentioned plasmid (figure 1).
As a bridge which connect the conjugation system and the self destructive plasmid, the time-delay genetic circuit as described above could not been constructed and tested until the other two sub-projects have been completed. To avoid a shift in the schedule, the device will be tested using IPTG as input and green fluorescent protein (GFP) as output (figure 2). A detailed scheme and description of this device will be showed in the following sections.