"
Team:Newcastle/Labwork/30 September 2009
From 2009.igem.org
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Contents |
Formal Lab Session - 30th September 2009
Chassis team
Introduction
- Since we got the Midi Prep of kinA transformation, we have enough plasmid which are enought for digestion test. We take Chris's advice to use new ladder which would be more accurate in measuring DNA's size.
Experiment procedure
Digestion
- Digestion reaction
dd H2O 7ul
10X fast digest buffer 2ul
Fast BamHI 0.5ul
Fast HindIII 0.5ul
Mini Prep DNA 10ul
------------------------------
20ul
- K1: kinA midi result digested by EcoRI and NheI
- K1: kinA midi result digested by HindIII
- Other three digest sample came from Jess's mini prep for sac transformation.
Conclusion
- Lane 3,4 were K1, K2 digested result. The kinA fragment is 2048bp, so the kinA band should be at the same position with the 6th band of lamda ladder or the 8th band of 1kb ladder. But the gel picture showed that those three bands were not at the same level. Even the ladder are not consist with each other. To confirm our kinA clone result, we need digested pMK-RQ as a standard control.
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
