Team:BIOTEC Dresden/Methods SilverNano

Silver Nano Particles - Methods

A ---- ml aliquot of the silver binding peptide ( sequence -----) was obtained from.... Two different experiments were setup using the following protocols.

Protocol 1:

A protocol that was initially used to identify metal binding peptides by was used to nucleate and grow silver nanoparticles[1].

• 120 ul peptide (8ug/ul)
• 14 ul HEPES buffer at pH 7.5
• 1.4 ul AgNO3 at 20mM
• 4.6 ul H2O

The solutions were mixed and incubated at room temperature for 24-48 hrs.

Protocol 2:

A method designed by Fromm and Wennemers [2] was used to form silver nanoparticles by using a chemical reducing agent, sodium ascorbate (NaAsc.)

• 120 ul peptide (8ug/ul)
• 14 ul NaAsc. at 2mM
• 1.4 ul AgNO3 at 20mM
• 4.6 ul H2O

[1] R.Naik et al., Adv. Funct. Mater., 14, 25-30, 2004
[2] K.Belser, K.Fromm, H.Wennemers et al., Angew. Chem. Int. Ed., 48, 3661-3664, 2009